Immobilization of Papain with Ionic Liquids-regenerated Cellulose Membrane for Wool Fabric Modification†

doi:10.7503/cjcu20150498

Abstract

Abstract:

Ionic liquid(IL) 1-butyl-3-methylimidazolium chloride([Bmim]Cl) was used to dissolve microcrystalline cellulose Avicel to make IL-regenerated cellulose membrane for papain immobilization through silane modification method. It demonstrated that the immobilized papain could be obtained at the condition of 6.5%(mass fraction) Avicel/[BMIM]Cl for membrane as the solid support; 30% NH₂(CH₂)₃Si(OC₂H₅)₃ at 50 ℃ to functionalize the carrier; adsorbing for 3 h followed by cross-linking for 4 h at 50 ℃, pH=7.0 and enzyme concentration of 300 mg/mL, using 10%(volume fraction) glutaraldehyde as the cross-linker. After immobilization, papain was immobilized to the both sides of the regenerated cellulose membrane surfaces with high loading density. The immobilized papain could be limited on the wool fiber surface, only digesting the scale structure instead of native papain penetrating inside the fiber and generating the significant weight and strengthen loss.

Key words: Papain, Immobilization, Ionic liquid, Cellulose, Scale of wool

CLC Number:

O636.1⁺1

TrendMD:

LI Qiujin, ZHAO Zhiqi, YUAN Yamei, GONG Jixian, ZHANG Jianfei. Immobilization of Papain with Ionic Liquids-regenerated Cellulose Membrane for Wool Fabric Modification^†[J]. Chem. J. Chinese Universities, 2015, 36(12): 2590.

Figures/Tables 5

Fig.1 TG(A) and DTG(B) analysis of Avicel(a) and [Bmim]Cl-regenerated cellulose membrane(b)

Fig.2 FTIR sprectra of original cellulose(a), [Bmim]Cl-regenerated cellulose membrane(b), and regenerated cellulose membrane after silane modification(c)

Fig.3 Effects of immobilization factors on activity of papain immobilized on [Bmim]Cl-regenerated cellulose membranes (A) Content of Avicel; (B) content of NH2(CH2)3Si(OC2H5)3; (C) temperature of silane modification; (D) pH; (E) adsorption time; (F) content of glutaraldehyde; (G) crosslinking time; (H) crosslinking temperature; (I) enzyme concentration.

Fig.4 SEM images of immobilized papain on Ils-regenerated cellulose membrane (A) Regenerated cellulose membrane; (B), (C) immobilized papain on regenerated cellulose membrane; (D)—(F) cross section of immobilized papain on regenerated cellulose membrane.

Fig.5 SEM images of wool fiber digested by immobilized papain (A) Original wool fiber; (B) wool fiber pretreated by H2O2; (C), (D) wool fiber hydrolyzed by free papain; (E), (F) wool fiber digested by immobilized papain.

References 37

[1]	Roy J., Sumi S., Sangeetha K., Abraham T., J. Chem. Technol. Biotechnol., 2005, 80(2), 184—188
[2]	Khaparde S. S., Singhal R. S., Bioresource Technol., 2001, 78(1), 1—4
[3]	Lei H., Wang W., Chen L. L., Li X. C., Yi B., Deng L., Enzyme Microb. Technol., 2004, 35(1), 15—21
[4]	Liang Y. Y., Zhang L. M., Biomacromolecules, 2007, 8(5), 1480—1486
[5]	Lin Z. B., Zhang H., Chen Q. D., Wan Y., Yang B., Su X. G., Zhang J. H., Jin Q. H., Chem. J. Chinese Universities,2003, 24(4), 609—611
	(林章碧, 张皓, 陈奇丹, 万异, 杨柏, 苏星光, 张家骅, 金钦汉. 高等学校化学学报,2003, 24(4), 609—611)
[6]	Wang M. F., Qi W., Su R. X., He Z. M., Chem. J. Chinese Universities,2010, 31(9), 1774—1779
	(王梦凡, 齐崴, 苏荣欣, 何志敏. 高等学校化学学报,2010, 31(9), 1774—1779)
[7]	Xiang G. Y., Lv Z. M., Eckstein H., Chem. J. Chinese Universities,2004, 25(10), 1853—1856
	(项光亚, 吕子敏, Eckstein H. 高等学校化学学报,2004, 25(10), 1853—1856)
[8]	Shen J. S., Rushforth M., Cavaco-Paulo A., Guebitz G., Lenting H., Enzyme Microb. Technol., 2007, 40(7), 1656—1661
[9]	Wang P., Wang Q., Cui L., Fan X. R., Yuan J. G., Gao M. R., Fibers Polym., 2010, 11(4), 586—592
[10]	El-Sayed H., El-Khatib E., J. Chem. Technol. Biotechnol., 2005, 80(10), 1111—1117
[11]	Zhang H., Deb-Choudhury S., Plowman J., Dyer J., J. Appl. Polym. Sci., 2013, 127(2), 3435—3440
[12]	Shen J., Bishop D., Heine E., Hollfelder B., J. Text. Inst., 1999, 90(3), 404—411
[13]	Silva C. L. S. M., Zhang Q. H., Shen J. S., Cavaco-Paulo A., Enzyme Microb. Technol., 2006, 39(4), 634—640
[14]	Silva C. L. S. M., Sousa F., Gübitz G., Cavaco-Paulo A., Food Technol. Biotechnol., 2004, 42(1), 51—56
[15]	Silva C. L. S. M., Gübitz G., Cavaco-Paulo A., J. Chem. Technol. Biotechnol., 2005, 81(1), 8—16
[16]	Turner M.B., Spear S. K., Holbrey J. D., Rogers R. D., Biomacromolecules, 2004, 5(4), 1379—1384
[17]	Lynd L. R., Weimer P. J., van Zyl W. H., Pretorius I. S., Microbiol. Mol. Biol. Rev., 2002, 66(3), 506—739
[18]	Falkowski P., Scholes R. J., Boyle E., Canadell J., Canfield D., Elser J., Gruber N., Hibbard K., Hogberg P., Linder S., Mackenzie F. T., Moore B., Pedersen T., Rosenthal Y., Seitzinger S., Smetacek V., Steffen W., Science, 2000, 290(5490), 291—296
[19]	Swatloski R. P., Spear S. K., Holbrey J. D., Rogers R. D., J. Am. Chem. Soc., 2002, 124(18), 4974—4975
[20]	Turner M. B., Spear S. K., Holbrey J. D., Daly D. T., Rogers R. D., Biomacromolecules, 2005, 6(5), 2497—2502
[21]	Bagheri M., Rodriguez H., Swatloski R. P., Spear S. K., Daly D. T., Rogers R. D., Biomacromolecules, 2008, 9(1), 381—387
[22]	Ince H., Akman S., Koklu U., Fresenius J. Anal. Chem., 1992, 342(7), 560—562
[23]	Fang H., Sheng J. P., Wu X. R., Food Mach., 2000, 6, 27—29
	(方焕, 生吉萍, 吴显荣. 食品与机械, 2000, 6, 27—29)
[24]	Antal M. J. Jr., Varhegyi G., Ind. Eng. Chem. Res., 1995, 34(3), 703—717
[25]	Liu C. F., Sun R. C., Zhang A. P., Qin M. H., Ren J. L., Wang X. A., J. Agric. Food Chem., 2007, 55(6), 2399—2406
[26]	Sun R. C., Fang J. M., Goodwin A., Lawther J. M., Bolton A. J., J. Agric. Food Chem., 1998, 46(7), 2817—2822
[27]	Deng Y. H., Qi D. W., Zhao D. Y., Deng C. H., Zhang X. M., Zhao D. Y., J. Am. Chem. Soc., 2008, 130(1), 28—29
[28]	Ren Q., Wu J., Zhang J., He J.S., Guo M. L.,Acta Polym. Sinica, 2003, (3), 448—451
	(任强, 武进, 张军, 何嘉松, 过梅丽. 高分子学报, 2003, (3), 448—451)
[29]	Li Q. J., Yin Y. L., Su R. X., Qi W., He Z. M., Acta Chim. Sin., 2009, 67(1), 88—92
	(李秋瑾, 殷友利, 苏荣欣, 齐崴, 何志敏. 化学学报, 2009, 67(1), 88—92)
[30]	Zhang H., Wu J., Zhang J., He J. S., Macromolecules, 2005, 38(20), 8272—8277
[31]	Swatloski R. P., Spear S. K., Holbrey J. D., Rogers R. D., J. Am. Chem. Soc., 2002, 124(18), 4974—4975
[32]	Anderson J. L., Ding J., Welton T., Armstrong D. W., J. Am. Chem. Soc., 2002, 124(47), 14247—14254
[33]	School of Life Sciences, Sun Yat-Sen University, The Introdution of Biochemical Technology, People’s Education Press, Beijing, 1978, 60
	(中山大学生物系生化微生物教研室. 生化技术导论, 北京: 人民教育出版社, 1978, 60)
[34]	Cheng F.L., Mo J. H., Dai X. Y.,Acta Polym. Sinica, 1999, (4), 417—421
	(程发良, 莫金垣, 戴晓云. 高分子学报, 1999, (4), 417—421)
[35]	Battaglini F., Bartlett P. N., Wang J. H., Anal. Chem., 2000, 72(3), 502—508
[36]	Huang W.T., Hu X. Z., Application Handbook Enzymes, Science and Technology Press, Shanghai, 1989, 113—116
	(黄文涛, 胡学智. 酶应用手册, 上海: 科学技术出版社, 1989, 113—116)
[37]	Migneault I., Dartiguenave C., Bertrand M. J., Waldron K. G., Biotechniques, 2004, 37(5), 790—802