Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique†

doi:10.7503/cjcu20180660

Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (4): 793.doi: 10.7503/cjcu20180660

• Physical Chemistry • Previous Articles Next Articles

Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†

HUANG Xiaolin^1,², XIE Huan^1,³, CAO Hong^1,^*(), JIN Hongjie^1,⁴, LI Chun⁵, WU Tinghua²

1. College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China
2. Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, China
3. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China
4. College of Life Sciences, Shihezi University, Shihezi 8320034, China
5. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

Received:2018-09-29 Online:2019-04-03 Published:2019-01-24
Contact: CAO Hong E-mail:chyf_ch@126.com
Supported by:
† Supported by the National Natural Science Foundation of China(No. 21266029) and the Natural Science Foundation of Zhejiang Province, China(No.LY17B060011).

Abstract

Abstract:

The magnetic core gel microspheres loaded ionic liquids(ILs) were prepared using Fe₃O₄ nanoparticles coated with chitosan with good biocompatibility(Fe₃O₄/CS) as magnetic response material. The composition, structure, morphology and magnetic properties of the prepared Fe₃O₄/CS nanoparticles and magnetic core gel microspheres loaded [BMIM]PF₆ were characterized by Fourier transform infrared spectroscopy(FTIR), scanning electron microscope(SEM), transmission electron microscope(TEM), X-ray diffraction(XRD), thermogravimetric analysis(TG) and vibrating sample magnetometer(VSM). And the magnetic core gel microspheres loaded [BMIM]PF₆ was applied to immobilize Penicillium purpurogenum Li-3 cells for the biosynthesis of glycyrrhetinic acid monoglucuronide(GAMG) from glycyrrhizic acid(GL) to realize the rapid recovery and reuse of the whole-cell biocatalyst and ILs. The results showed that the Fe₃O₄ nanoparticles were successfully encapsulated by chitosan. And due to the uniform distribution of magnetic particles in the prepared gel microspheres loaded ILs, the microspheres show good magnetic properties. Compared with immobilized cells with gel microsphere(Ic) catalytic system, the yield of GAMG in immobilized cells with magnetic core gel microspheres loaded [BMIM]PF₆(IcMILs) catalytic system increased by 13.8%. The reutilization experiment showed that the IcMILs were easier to be recovered quickly under the applied magnetic field. After 9 cycles of recycling, the IcMILs relative activity still remained 59.2%. The preparation technique of the magnetic core gel microspheres loaded ILs has the potential to be further developed and applied in the rapid on-line separation and continuous operation of biocatalyst and ILs to increase the catalytic yield and reduce the cost.

Key words: Fe₃O₄/CS nanoparticles, Magnetic core gel microspheres loaded ionic liquid, [BMIM]PF₆, Cells immobilization technique, Biocatalysis

CLC Number:

O643.3

TrendMD:

HUANG Xiaolin,XIE Huan,CAO Hong,JIN Hongjie,LI Chun,WU Tinghua. Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†[J]. Chem. J. Chinese Universities, 2019, 40(4): 793.

Figures/Tables 8

Fig.1 TEM images of Fe3O4(A) and Fe3O4/CS(B)

Fig.2 SEM images of magnetic gel beads(A) and magnetic supported ionic liquid [BMIM]PF6 gel beads(B—F)Volume fraction of [BMIM]PF6: (A) 0; (B) 3%; (C) 6%; (D) 9%; (E) 12%; (F) 15%.

Fig.3 FTIR spectra of the chitosan(a), Fe3O4/CS(b), Fe3O4(c), magnetic supported [BMIM]PF6 gel beads(d) and [BMIM]PF6(e)

Fig.4 TG curves of Fe3O4(a), Fe3O4/CS(b) and magnetic supported [BMIM]PF6 gel beads(c)

Fig.5 XRD patterns of Fe3O4(a), Fe3O4/CS(b) and magnetic gel beads(c)

Fig.6 VSM of Fe3O4(a), Fe3O4/CS(b) and magnetic gel beads(c)

Fig.7 Yields of GAMG catalysed by Ic(a), IcMILs(b) and free cell(c)

Fig.8 Repeated utilization of magnetic immobilized cells supported with [BMIM]PF6

References 25

[1]	Itoh T., Chem.Rev., 2017, 117(15), 10567—10607
[2]	Sharma V. K., Mukhopadhyay R., Biol.Rev.,2018, 10(5), 1—14
[3]	Yao N., Wu Y. P., Zheng K. B., Hu Y. L., Currt. Org.Chem.,2018, 22(5), 462—484
[4]	Zhang W., Yue Y., Su W., Du W., Wang X., Catal.Commun.,2015, 65, 113—116
[5]	Elgharbawy A. A., Riyadi F. A., Alam M. Z., Moniruzzaman M., J. Mol.Liq.,2018, 251, 150—166
[6]	Fan L. L., Li H. J., Chen Q. H., Int. J. Mol.Sci.,2014, 15(7), 12196—12216
[7]	Fu M. L., Liu J., Dong Y. C., Yu F., Fang R. S., Chen Q. H., Liu X. J., Eur. Food Res.Technol.,2011, 233(3), 507—515
[8]	Sun L. H., Shen A. P., He B. L., Zheng Y. G., Journal of Chinese Institute of Food Science and Technology,2015, 15(2), 21—27
	(孙丽慧, 沈爱萍, 何宝龙, 郑裕国. 中国食品学报, 2015, 15(2), 21—27)
[9]	Gu Y. H., Xue P., Chem. J. Chinese Universities,2018, 39(8), 1846—1852
	(谷耀华, 薛屏. 高等学校化学学报, 2018, 39(8), 1846—1852)
[10]	Jyoti Bhatia K., Chauhan K., Attri C., Seth A., Int. J. Biol.Macromol.,2017, 103, 8—15
[11]	Cao H., Ye H., Li C., Zheng L. L., Li Y., Ouyang Q. F., Process Biochem.,2014, 49(5), 791—796
[12]	Li Y., Cao H., Ouyang Q. F., Li C., Zheng L. L., Zhang X., Chem. J. Chinese Universities,2014, 35(5), 1057—1062
	(李扬, 曹红, 欧阳巧凤, 李春, 郑兰兰, 张馨. 高等学校化学学报, 2014, 35(5), 1057—1062)
[13]	Pospiskova K., Safarik I., Carbohyd.Polym.,2013, 96(2), 545—548
[14]	Wang X. Y., Jiang X. P., Li Y., Zeng S., Zhang Y. W., Int. J. Biol.Macromol.,2015, 75, 44—50
[15]	Li Y., Wang X. Y., Zhang R. Z., Zhang X. Y., Xu X. M., Zhang Y. W., J. Nanosci.Nanotechno.,2014, 14(4), 2931—2936
[16]	Li Y., Wang X. Y., Jiang X. P., Ye J. J., Zhang Y. W., Zhang X. Y., J. Nanopart.Res.,2015, 17, 1—12
[17]	Liu W., Zhou F., Zhang X. Y., Li Y., Wang X. Y., Xu X. M., Zhang Y. W., J. Nanosci.Nanotechno.,2014, 14(4), 3068—3072
[18]	Zhang Q. K., Kang J. Q., Yang B., Zhao L. Z., Hou Z. S., Tang B., Chin. J.Catal.,2016, 37(3), 389—397
[19]	Pospiskova K., Prochazkova G., Safarik I., Lett. Appl.Microbiol.,2013, 56(6), 456—461
[20]	Safarik I., Pospiskova K., Maderova Z., Baldikova E., Horska K., Safarikova M., Yeast,2015, 32(1), 239—243
[21]	Li T., Liu Y., Peng Q., Hu X. J., Liao T., Wang H., Lu M., Chem. Eng.J.,2013, 214(4), 189—197
[22]	Berovic M., Berlot M., Kralj S., Makovec D., Biochem. Eng.J.,2014, 88(28), 77—84
[23]	Safarik I., Maderova1 Z., Pospiskova K., Baldikova1 E., Horska1 K., Safarikova M., Yeast,2015, 32(1), 227—237
[24]	Zheng L. L., Cao H., Tang M. X., Ouyang Q. F., Zhang X., J. Instrumental Anal.,2014, 33(7), 815—819
	(郑兰兰, 曹红, 唐明翔, 欧阳巧凤, 张馨. 分析测试学报, 2014, 33(7), 815—819)
[25]	Gregorio-Jauregui K. M., Pineda M. G., Rivera-Salinas J. E., Hurtado G., Saade H., Martinez J. L., J.Nanomater.,2012, 2012(2), 1—8

Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^†

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 25

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	SUN Taiqiang, LI Bin, NIE Yao, WANG Dong, XU Yan. Asymmetric Synthesis of (S)-N,N-Dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine by Cell-free System of Carbonyl Reductase CR2^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1772.
[2]	WANG Ke, WU Xia-Qin, LI Lin, TIAN Hai-Tao, LU Zhong-Qing. Electrochemical Catalytic Chlorination of Bergenin by Chloroperoxidase Modified Electrode [J]. Chem. J. Chinese Universities, 2013, 34(7): 1739.
[3]	GUO Can-Cheng, LI Zhi-Peng, LIANG Ben-Xi. Hammett Relationship in Cyclohexane Hydroxylation Catalyzed by Metalloporphyrins [J]. Chem. J. Chinese Universities, 1997, 18(2): 242.
[4]	Qi De-yao, Zhuang Yun-long, Chi Qi-jin. Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acid [J]. Chem. J. Chinese Universities, 1990, 11(12): 1349.