Synthesis of a Silica-bonded Bovine Serum Albumin Carbonyl Chiral Stationary Phase for High-performance Liquid Chromatographic Resolution of Enantiomers

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (7): 1251.

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Synthesis of a Silica-bonded Bovine Serum Albumin Carbonyl Chiral Stationary Phase for High-performance Liquid Chromatographic Resolution of Enantiomers

FENG Shun^1,2, ZHANG Qiang², ZOU Han-Fa², WANG Ji-De¹, CHEN Xiao-Ming², ZHANG Zeng-Zi²

1. Department of Chemistry, Xinjiang University, Urumqi 830024, China;
2. National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China

Received:2001-08-20 Online:2002-07-24 Published:2002-07-24

Abstract

Abstract: An HPLCchiral stationary phase was synthesized by the in situ covalent immobilization of bovine serum albumin on a aminopropyl silica packed column activated with 1, 1 carbonyldiimidazole in a short time. The effects of mobile phase pHand the content of 1 propanol on k' and α of D, L-trypto-phan were investigated, and the resolution of seven chiral compounds was successfully achieved.

Key words: Chiral separation, Bovine serum albumin, Aminopropyl silica gel, 1,1-Carbonyldiimidazole, Chiral stationary phase

CLC Number:

O657.7

TrendMD:

FENG Shun, ZHANG Qiang, ZOU Han-Fa, WANG Ji-De, CHEN Xiao-Ming, ZHANG Zeng-Zi . Synthesis of a Silica-bonded Bovine Serum Albumin Carbonyl Chiral Stationary Phase for High-performance Liquid Chromatographic Resolution of Enantiomers[J]. Chem. J. Chinese Universities, 2002, 23(7): 1251.

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Synthesis of a Silica-bonded Bovine Serum Albumin Carbonyl Chiral Stationary Phase for High-performance Liquid Chromatographic Resolution of Enantiomers

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