The Behavior of Components Transfer in Chip-based Capillary Electrophoresis and Its Characteristics

Abstract

Abstract: On the home made microchip capillary electrophoresis system with laser induced fluorescent(LIF) detection, the electrophoresis behavior of single dye and amino acids labeled with fluorescein 5-isothiocyanate(FITC) was demonstrated on glass chips. The comparison between chip-based electrophoresis and conventional capillary electrophoresis was taken into account. The special attention was paid for the characteristic of chip-based electrophoresis. The effect of various of operation conditions, such as countervoltage, sample loading modes and point of detection, were investigated.

Key words: Microchip, Laser-induced fluorescence detection, Capillary electrophoresis, Amino acids

CLC Number:

O658

TrendMD:

WANG Hui, MAO Xiu-Li, GAI Hong-Wei, BAI Ji-Ling, LIN Bing-Cheng . The Behavior of Components Transfer in Chip-based Capillary Electrophoresis and Its Characteristics[J]. Chem. J. Chinese Universities, 2002, 23(6): 1030.

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The Behavior of Components Transfer in Chip-based Capillary Electrophoresis and Its Characteristics

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