Characteristics of Bioelectrochemical Response ofan Amine Oxidase-modified Polyaniline Electrode

Abstract

Abstract: An amine oxidase-modified polyaniline electrode(PANI/AO) was formed by immobilization ofamine oxidase in polyaniline film by electrochemical doping method.The electrode has a fast response forhistamine and the reaction is controlled by the enzyme kinctics.The apparent Michaelis constant, optimunpHand activation energy for the immobilized amine oxidase are 0.21 mmol/L, 7.3-7.6 and 76kJ/mol,respectively.The enzyme electrode exhibited a good stability, which can be used for the determination ofhistamine.In addition, the electrochemical characteristics of the enzyme electrode were studied by cyclicvoltammetry and impedance measurement.

Key words: Polyaniline, Amine oxidase, Biosensor, Enzyme electrode, Histamine determination

CLC Number:

O631

TrendMD:

XUE Huai-Guo, SHEN Zhi-Quan, LI Yong-Fang. Characteristics of Bioelectrochemical Response ofan Amine Oxidase-modified Polyaniline Electrode[J]. Chem. J. Chinese Universities, 2002, 23(4): 730.

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Characteristics of Bioelectrochemical Response ofan Amine Oxidase-modified Polyaniline Electrode

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