Abstract: A simple and valid procedure for the preparation of open tubular electrochromatography column with derivated polysiloxane as stationary phase was established. When using polysiloxane derivated with butyl methacrylate and vinylsulfonic acid as stationary phase, the column has a strong ability for separation of the series of benzene. The separation efficiency of tuluene was 1.52×10⁵ theoretical plates/meter and the retention factors of the series of benzene were larger than 2.40 After two months, there was no obvious difference in the separation ability of the column. In this method, the polymer was directly bonded in 50 μm i.d. fused silica capillary without pre etching. When using polysiloxane derivated with allyl-permethyl-β-cyclodextrin and vinylsulfonic acid as chiral stationary phase, naproxen could be enantiomeric separated with resolution of 0.81. In this method, the 50 μm i.d. fuse-silica capillary was primarily treated with Sol-Gel technology to increase the area of inner wall and the density of Si-OH.

Key words: Capillary electrophoresis, Open tubular electrochromatography column, Derivated polysiloxane