Abstract: Amicellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm×50 μm i.d. capillary tube using a 300 mmol/L SDS-25 mmol/Lborate-50 mmol/Lphosphate buffer (pH=6.96), 0.5 Psi×15 s injection, 7.0 kVvoltage and UVdetection at 254 nm. By using the MEKCmethod developed, 14 normal and modified nucleosides in 28 normal urines were quantified to establish the reference ranges. The result shows that this MEKCmethod is reliable, sensitive, efficient and suitable for clinical application.

Key words: Micellar electrokinetic chromatography, Nucleosides, Urine