Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (1): 34.

• Articles • Previous Articles     Next Articles

Studies on Quantum Dots-labeled Trichosanthin

ZHANG Chun-Yang1, MA Hui1, DING Yao1, JIN Lei1, CHEN Die-Yan1, MAO Pi2, NIE Shu-Ming3   

  1. 1. Educational Ministry Key Laboratory of Molecular and Nano Sciences, Department of Physics, Tsinghua University, Beijing 100084, China;
    2. National Laboratory of Biomacromolecular, Institute of Biophysics, Academia Sinica, Beijing 100101, China;
    3. Departmentof Chemistry of Indiana University, USA
  • Received:2000-03-22 Online:2001-01-24 Published:2001-01-24

Abstract: Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCSvaried to a less extent with wavelength in the range of 400-600 nm. The fluorescent spectrum of QDs-TCSunderwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCSin comparison with trichosanthin. Distribution of QDs-TCSwithin human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCScould enter the cytoplasm across the membrane and aggregated around the nuleolus.

Key words: Quantum dots, Trichosanthin, Enzymatic activevity

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