Effect of Chelate Reagents on Terbium-apotransferrin

Abstract

Abstract: The effect of chelate reagents on Tb³⁺-transferrin was studied at room temperature and pH7.4. The results show that the binding of Tb³⁺to apotransferrin is reversible in 0.1 mol/L N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid and pH7.4. Ethylenediamine tetraacetic acid, and nitrilotriacetic acid can make Tb³⁺-transferrin dissociated completely. The species of Tb³⁺in serum was calculated by using ECCLES program. Tbc-trans-ferrin is 21%, Tb-citrate is 62%, Tb-Asp is 15% when terbium concentration of Tb³⁺is smaller than 5×10^-5mol/L.

Key words: Transferrin, Chelate reagent, Species, Terbium(Ⅲ)

CLC Number:

O643

TrendMD:

ZHANG Ai-Ping, YANG Bin-Sheng . Effect of Chelate Reagents on Terbium-apotransferrin[J]. Chem. J. Chinese Universities, 1999, 20(7): 1106.

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Effect of Chelate Reagents on Terbium-apotransferrin

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