Enhancement of Glucose Biosensor Sensitivity by Addition of Silver Sols

Abstract

Abstract: Anew method for preparation of amperometric glucose oxidase electrode based on enzyme immobilization by Ag sols has been investigated. GOD was simply mixed with Ag nanoparticles and cross linked with a polyvinyl butyl(PVB) medium by glutaradehyde and was coatted on electrode by dipping a platinum wire into the sol-gel solution. In experiments, we discussed the effects of different kinds of silver gels on response of the electrode and the mechanism about it. For the optimum conditions, the current response of the enzyme electrode containing hydrophobic silver gels for 10 mmol/Lglucose was enhanced to 18200 nAas compared with the electrode without silver gels with 280n Acurrent response. In addition to the high sensitivity, the electrode responses rapidly, the 95% steady-state response time is less than 20s.

Key words: Silver nanoparticle, Glucose oxidase, High sensitivity, Rapid response

CLC Number:

O657.3

TrendMD:

TANG Fang-Qiong, SHEN Ji-Feng, ZHANG Jin-Fang, ZHANG Gai-Lian . Enhancement of Glucose Biosensor Sensitivity by Addition of Silver Sols[J]. Chem. J. Chinese Universities, 1999, 20(4): 634.

[1]	YANG Zhaohua, CHENG Hongjing, YANG Yi, LIU Hui, DU Feipeng, ZHANG Yunfei. Preparation of Silver-loaded Polyvinyl Alcohol Sponge and Its Interfacial Photothermal Driven Water Evaporation Performance [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220181.
[2]	CHEN Shaoyun, ZHANG Xingying, LIU Ben, TIAN Du, LI Qi, CHEN Fang, HU Chenglong, CHEN Jian. Controllable Growth of Silver Nanoparticles on TiO₂ Tetragonal Prism Nanarrays and Its SERS Effect [J]. Chem. J. Chinese Universities, 2021, 42(8): 2381.
[3]	LI Liu, SUN Shiyong, LYU Rui, GOLUBEV Yevgeny Aleksandrovich, WANG Ke, DONG Faqin, DUAN Tao, KOTOVA Olga Borisovna, KOTOVA Elena Leonidovna. Construction of Fe-aminoclay-glucose Oxidase Nanocomposite Catalyst and Its Multi-enzyme Cascade Analysis [J]. Chem. J. Chinese Universities, 2021, 42(3): 803.
[4]	WANG Huan, SUO Jinquan, WANG Chunyan, WANG Runwei. Glucose Oxidase Immobilization with Amino Dendritic Mesoporous Silica Nanoparticles and Its Application in Glucose Detection [J]. Chem. J. Chinese Universities, 2020, 41(8): 1731.
[5]	WEI Simin,WANG Yinghui,TANG Zhishu,SU Rui,HU Jinhang,GUO Hui,LI Chen,JIANG Jintao,SONG Zhongxing. Novel Biosynthesis Method of Silver Nanoparticle by UV Radiation of Cornus Officinalis Aqueous Extract and Biological Activities ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1391.
[6]	FENG Wei,WANG Bowei,JIANG Yang,LI Longyun. Design, Preparation and Surface-enhanced Raman Scattering(SERS) Spectrum of Single Ag Nanodot^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1345.
[7]	WANG Wen,TAO Xiafang,WU Yunyan,ZHAO Nan,CHENG Xiaonong,YANG Juan,ZHOU Yazhou. Fabrication and SERS Performance of “Sandwich” Structured Silver Nanoparticles/Graphene Oxide Substrates^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 667.
[8]	XIAO Shanshan, OUYANG Yiting, LI Xiaoyun, WANG Zhao, WU Pan, DENG Zhao, CHEN Lihua, SU Baolian. Synthesis of a Core-shell Structured Ag/ZIF-8 Catalyst with Mesoporous Silica Shell^† [J]. Chem. J. Chinese Universities, 2018, 39(6): 1235.
[9]	WANG Hongmei, LI Lingling, CHEN Haibin, ZENG Yongming, CHEN Hongju, CHEN Qizhen, LIN Huizhen, TIAN Zhongqun, LIU Guokun. Application of Surface-enhanced Raman Spectroscopy in the Fast Screening of Phosphodiesterase Type 5 Inhibitors^† [J]. Chem. J. Chinese Universities, 2017, 38(6): 1040.
[10]	HUANG Haiping, YUE Yafeng, XU Liang, LÜ Lianlian, HU Yongmei. Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 554.
[11]	FENG Juanjuan, ZHAO Yiman, WANG Haiyan. Colorimetric Detection of Dopamine Based on Silver Nanoparticles^† [J]. Chem. J. Chinese Universities, 2015, 36(7): 1269.
[12]	WANG Ping, NI Yong-Nian. Silver Nanoparticles Preparation Using Antioxidant Propyl Gallate and Its Analytical Application [J]. Chem. J. Chinese Universities, 2013, 34(4): 837.
[13]	WU Jian, ZHENG Yu-Dong, GAO Shuang, GUO Jia, CUI Qiu-Yan, DING Xun, CHEN Xiao-Hua. Synthesis, Structural Characteristics and Properties of Silver Nanoparticles in situ Bacterial Cellulose Gelatinous Membrane [J]. Chem. J. Chinese Universities, 2013, 34(1): 210.
[14]	XU Miao-Jun, CHEN Tian-Ran, LI Bin. Effective Fabrication of Silver Nanobowl Array Structure and Its Enhanced Fluorescence Property [J]. Chem. J. Chinese Universities, 2012, 33(11): 2368.
[15]	GUO Hong-Yun, XU Shu-Ping, TANG Bin, TAO Jin-Long, PAN Ling-Yun, XU Wei-Qing. Construction of Composite Surface-enhanced Raman Scattering (SERS) Substrates by Silver Nanoparticle Assembly [J]. Chem. J. Chinese Universities, 2012, 33(10): 2308.

Enhancement of Glucose Biosensor Sensitivity by Addition of Silver Sols

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments