Studies on the Reaction Between Zincon and Bovine Serum Album in

Abstract

Abstract: The interaction of zincon(ZCN) with bovine serum albumin(BSA) and the changes of its spectrum were studied by UV spectra at pH4.1 buffer solution. The conditional constants, apparent molar absorptivity ε_P=1.3×10⁶L·mol^-1·cm^-1, maximum binding number n=278 and apparent binding equilibrium constant K_c=8×10⁷were obtained. It was considered that electrostatic force is the main binding force. The ion(sodium chloride) concentration of the solution has significant effect on binding reaction of BSAand ZCN. The effects of different denaturants were also examined. The Scatchard model is appropriate in the treatment of data obtained.

Key words: Zincon, Bovine serum albumin, UV spectra, Apparent binding equilibricem constant, Denaturant

CLC Number:

O657

TrendMD:

CHI Yan-Hua, ZHUANG Jia, LI Na, LI Ke-An, TONG Shen-Yang . Studies on the Reaction Between Zincon and Bovine Serum Album in[J]. Chem. J. Chinese Universities, 1999, 20(11): 1697.

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Studies on the Reaction Between Zincon and Bovine Serum Album in

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