Application in the Damage of DNA by Electrochemical DNA Sensor

Abstract

Abstract: Mutation and damage in DNAby interaction, UV-irralation, nitrite action and ultrasonication were studied by means of voltammetry using a graphite electrode covalently immobilied with ssDNA-1. Daunomycin was used as a hybidization indicator. When the complementary ssDNA-2 in solution was damaged by phsical or chemical agents, the hybridization of ssDNA-1 and ssDNA-2 was changed. All the results in voltammogram indicated that intercalator can't destroy the double-helical structure of DNA, while UV-irralation and nitrite action can make the permanent damage. Ultrasonication can reduce polymerization in DNAand the hybridization time was shortened. This may provide a new method for clinical medicine study.

Key words: DNA, Electrochemical sensor, Daunomycin, Hybridization of nucleic acid molecules, Voltammetry

CLC Number:

O657.1

TrendMD:

SUN Xing-Yan, XU Chun, LIU Sheng-Hui, HE Pin-Gang, FANG Yu-Zhi . Application in the Damage of DNA by Electrochemical DNA Sensor[J]. Chem. J. Chinese Universities, 1998, 19(9): 1393.

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Application in the Damage of DNA by Electrochemical DNA Sensor

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