A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy

Abstract

Abstract: The properties of Hemoglobin adsorbed on the silver colloid surface were investigated by using surface enhanced Raman spectroscopy(SERS) in the determination of oxygen-hemoglobin, deoxygen-hemoglobin and heme. The results show that the heme chromophore of oxygen-hemoglobin interacts directly with silver colloid, as a result of either its detachment from the protein matrix or denaturation from the protein. The deoxygen-hemoglobin would not release its heme at the silver colloid as normally expected.

Key words: Hemoglobin, Heme, Surface enhanced Raman spectroscopy

CLC Number:

O657.37

TrendMD:

YE Bao-Xian, LIN Lin, ZHANG Wu-Ming, ZHOU Xing-Yao. A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy[J]. Chem. J. Chinese Universities, 1998, 19(8): 1239.

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A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy

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