NaNO2 Biosensor Based on Hollow Nano-nickel Oxide Microsphere and Ionic Liquid Composite Film Immobilizing Hemoglobin

doi:10.3969/j.issn.0251-0790.2012.09.009

Abstract

Abstract:

Hollow nano-nickel oxide microsphere (NiO) was fabricated by microwave hydrothermal process and characterized by SEM. The prepared NiO was used to immobilize hemoglobin(Hb) on the surface of the carbon paste electrode with 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF₄). The structure of Hb in the composite film was still maintained native-like demonstrated by FTIR spectra and UV-visible spectra. Moreover, the functions of different components in the modified electrode were studied by electrochemical impedance spectroscopy. A pair of stable and quasi-reversible redox peaks was observed in phosphate buffer solution which indicating that heme-proteins could achieve its direct electron transfer effectively. The modified electrode displayed an excellent and rapid electrocatalytic response to the oxidation of NaNO₂. The proposed biosensor exhibited a good linear response to the concentration of NaNO₂ in the range of 10-170 μmol/L, with a detection limit of 4.57 μmol/L(S/N=3). The apparent Michaelis-Menten constant was estimated to be 2.4 mmol/L. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Key words: Hollow nano-nickel oxide microsphere, Hemoglobin, Direct electron transfer, NaNO₂, Biosensor

CLC Number:

O657.1

TrendMD:

GU Guang-Zhe, DONG She-Ying, HUANG Ting-Lin, TANG Hong-Sheng, ZHENG Jian-Bin. NaNO₂ Biosensor Based on Hollow Nano-nickel Oxide Microsphere and Ionic Liquid Composite Film Immobilizing Hemoglobin[J]. Chem. J. Chinese Universities, 2012, 33(09): 1926.

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NaNO₂ Biosensor Based on Hollow Nano-nickel Oxide Microsphere and Ionic Liquid Composite Film Immobilizing Hemoglobin

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