Studies of Interaction of Phenazine Dye and DNA by UV-Vis Spectrum

Abstract

Abstract: The solution state of Neutral Red(NR) with or without DNAwas investigated by UV-Vis spectrum. NRmolecules are dimerized in water. The NRabsorbance spectra of monomer and dimer are obtained by calculating and the calculated dimerization equilibrium constant Kis 8.4×10^{4>/sup> L·mol^-1 . NR and DNA strongly interact with each other. The apparent binding constant(K_ap) and the binding numbers of NRand DNAfrom absorption spectra data are 5.79×10⁴ L·mol^-1 and 43, respectively. Both the influence of ionic strength on the interaction between N Rand DNA and NR on T_m of DNAare also studied.}

Key words: Solution state, Intercalation, DNA, Phenazine dye

CLC Number:

O657.32

TrendMD:

CAO Ying, HE Xi-Wen  . Studies of Interaction of Phenazine Dye and DNA by UV-Vis Spectrum[J]. Chem. J. Chinese Universities, 1998, 19(5): 714.

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Studies of Interaction of Phenazine Dye and DNA by UV-Vis Spectrum

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