Studies on the Separation and Extraction of α-Amylase and Proteinase from Fermented Liquor of α-Amylase by Aqueous Two-phase Partitioning

Abstract

Abstract: Aqueous two-phase system composed of PEG/(NH₄)₂SO₄ was once to isolate α-amylase and proteinase from fermented liquor of α-amylase in this paper. The optimum extraction conditions were: 15% PEG1000, 20% (NH₄)₂SO₄, pH=8, the partition coefficient of α-amylase was as high as 19.6 with a yield of 90%. The separation coefficients between α-amylase and proteinase were 15.1 and 1.5 folds increase of specific activity compared with those the original fermented liquor. The yield of proteinase in water phase was more than 60%. The preliminary study on back-extraction condition was done in this paper.

Key words: α-Amylase, Proteinase, Aqueous two-phase system, Polyethylene glycol, Extraction, Back-extraction

CLC Number:

O629

TrendMD:

JIAO Qing-Cai, LIU Qian, CHEN Yao-Zu. Studies on the Separation and Extraction of α-Amylase and Proteinase from Fermented Liquor of α-Amylase by Aqueous Two-phase Partitioning[J]. Chem. J. Chinese Universities, 1998, 19(3): 391.

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Studies on the Separation and Extraction of α-Amylase and Proteinase from Fermented Liquor of α-Amylase by Aqueous Two-phase Partitioning

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