Determination of DNA by a Self-quenching Fluorescence Probe

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (9): 1456.

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Determination of DNA by a Self-quenching Fluorescence Probe

ZHAO Yi-Bing, WANG Dong-Yuan, XU Ke-Wei, GUO Xiang-Qun, XU Jin-Gou

Department of Chemistry, The Research Laboratory of SEDC of Analytical Sciencefor Material and Life Chemistry, Xiamen University, Xiamen 361005

Received:1996-12-12 Online:1997-09-24 Published:1997-09-24

Abstract

Abstract: This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNArevealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15 ng/mL 6.0μg/mL.

Key words: Self-quenching, Fluorescence probe, DNA

CLC Number:

O657.39

TrendMD:

ZHAO Yi-Bing, WANG Dong-Yuan, XU Ke-Wei, GUO Xiang-Qun, XU Jin-Gou. Determination of DNA by a Self-quenching Fluorescence Probe[J]. Chem. J. Chinese Universities, 1997, 18(9): 1456.

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Determination of DNA by a Self-quenching Fluorescence Probe

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