Voltammeric Determination of DNA with Doxorubicin Hydrochlorldum as Intercalator on Graphite Electrode

Abstract

Abstract: Asynthesized 75-mer single stranded DNA (ssDNA-1) was covalently immobilized on a oxidized graphite electrode surface that had been activated using EDCand NHS.The immobilized ssDNA-1 hybridized with various concentrations of complementary ssDNA-2 in hybridization buffer containing DXH.The dsDNA/DXHsystem was formed on the electrode surface.The cathodic waves of the DXHbound to the dsDNAwith cyclic voltammetry were used for the determination of ssDNA-2.The reduction peak currents of DXHwere linearly related to the concentration of ssDNA-2 between 1×10^-5 to 5×10^-7 mg/mL.The determination demonstrated the method is practicable.

Key words: DNA, Intercalator, Doxorubicin hydrochloridum, Voltammetry

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FANG Yu-Zhi, LIU Sheng-Hui, HE Pin-Gang. Voltammeric Determination of DNA with Doxorubicin Hydrochlorldum as Intercalator on Graphite Electrode[J]. Chem. J. Chinese Universities, 1996, 17(8): 1222.

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Voltammeric Determination of DNA with Doxorubicin Hydrochlorldum as Intercalator on Graphite Electrode

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