N-Hydroxysuccinimidyl-α-naphthylacetate as Pre-cloumn Derlvating Reagent to Scperate and Determine Peptides and Their Hydrolysates by Reversed Phase HPLC

Abstract

Abstract: Using N-hydroxysuccinimidyl-α-naphthylacetate(SINA,synthesized by ourselves) as pre-column derivating reagent,glutathion,diglxcyl-glycine,glycyl glycine, Glu,(Cys)₂ and Gly were separated and determined by reversed phase high performance liquid chromatograph (RP-HPLC).The six derivatives were eluted within 16min with a mobile phase of methanol-acetic ether-water(17/3/80 ,volume ratio)containing 15 mmol/L pH=5.4 acetate buffer on C18 column,At the ratio of signal to noise S/N=3,the detection limit is in lower picornole range,The reproducibility was good.

Key words: High performance liquid chromatograph, N-Hydroxysuccinimidyl-α-naphthylacetate, peptides and their hydrolysates

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ZHAO Yuan-Yuan, XU Guo-Liang, ZHANG Hua-Shan, LI Jun. N-Hydroxysuccinimidyl-α-naphthylacetate as Pre-cloumn Derlvating Reagent to Scperate and Determine Peptides and Their Hydrolysates by Reversed Phase HPLC[J]. Chem. J. Chinese Universities, 1996, 17(7): 1044.

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N-Hydroxysuccinimidyl-α-naphthylacetate as Pre-cloumn Derlvating Reagent to Scperate and Determine Peptides and Their Hydrolysates by Reversed Phase HPLC

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