Rapid Separation of Ginsenosides Based on Multi-walled Carbon Nanotubes†

doi:10.7503/cjcu20180280

Abstract

Abstract:

Multi-walled carbon nanotubes(MWCNTs) were employed as the selective adsorbent to investigate their performance in the rapid separation of ginsenoside from ginseng extract. Ginseng was ultrasonically extracted in aqueous methanol. The resulting extract mainly consisted of ginsenoside and saccharide. Saccharide in ginseng was similar with ginsenoside in polarity, and was the main interfering component during the extraction and separation of ginsenoside. Therefore, it is essential to separate saccharide from ginsenoside in ginseng extract. MWCNTs could rapidly adsorb and desorb ginsenoside in ginseng extract, but not the saccharide. Based on this selective adsorption, a novel method using MWCNTs for the rapid separation of ginsenoside and saccharide was developed. Under the optimized separation conditions, MWCNTs had high degree of saccharide separation and ginsenoside recovery, both of which were more than 90%, and also had high adsorption capacity of 15.0—24.0 μg/mg to the 8 main ginsenosides, Rb1, Rb2, Rc, Rd, Re, Rg1, 20(S)-Rf and Ro. Further research revealed that the 8 ginsenosides could reach the adsorptive and the desorptive equilibrium within 3 min, and their recovery were significantly affected by the polarity of desorption solvent. The short equilibrium time was the foundation of the rapid separation of ginsenoside. Comparing with conventional material used for the separation of ginsenoside, such as macroporous resin, MWCNTs-based separation method is less time-consuming, more convenient and efficient.

Key words: Multi-walled carbon nanotubes, Ginsenoside, Rapid separation, High performance liquid chromatography-mass spectrometry(HPLC-MS)

CLC Number:

O657

TrendMD:

MIAO Rui,WU Dongxue,WANG Qiuying,ZHAO Huanxi,LI Xue,XIU Yang,LIU Shuying. Rapid Separation of Ginsenosides Based on Multi-walled Carbon Nanotubes^†[J]. Chem. J. Chinese Universities, 2018, 39(10): 2178.

Figures/Tables 9

Table 1 Validation of the developed HPLC-QqQ/MS method used for the quantification of the 8 ginsenosides

Ginsenoside	Calibration curve	R²	Linear range/ (μg·mL^-1)	LOD/ (μg·mL^-1)	LOQ/ (μg·mL^-1)	Repeatability (RSD, %)	Recovery(%)/RSD(n=3, %)
Ginsenoside	Calibration curve	R²	Linear range/ (μg·mL^-1)	LOD/ (μg·mL^-1)	LOQ/ (μg·mL^-1)	Repeatability (RSD, %)	80%	100%	120%
Re	y=16733.09x+4922	0.995	0.5—200.0	0.09	0.31	2.86	103.12/1.40	98.24/3.65	96.38/3.61
Rg1	y=24013.13x+510.99	0.998	0.5—200.0	0.12	0.40	3.75	99.32/2.55	95.43/2.15	101.64/1.88
20(S)-Rf	y=3428.46x+930.66	0.998	0.5—200.0	0.10	0.34	2.23	93.54/2.77	108.75/1.23	89.45/2.43
Rb1	y=6777.37x+3176.51	0.997	0.5—200.0	0.14	0.45	2.61	108.71/4.27	95.01/2.53	92.17/3.40
Rb2	y=13982.11x+2949.74	0.994	0.5—200.0	0.13	0.44	1.08	101.55/2.43	98.34/3.54	95.35/1.88
Rc	y=12188.45x-4503.28	0.997	0.5—200.0	0.14	0.45	3.47	89.16/3.58	93.15/3.16	103.34/4.88
Rd	y=23267.82x-945.67	0.997	0.5—200.0	0.14	0.47	3.89	95.37/2.19	92.76/3.63	93.32/2.38
Ro	y=15753.62x+983.03	0.994	0.5—200.0	0.10	0.35	3.15	106.24/2.75	88.67/5.34	97.52/4.36

Fig.1 Base peak intensity chromatograms of ginseng extract(A), ginseng extract after being absorbed by MWCNTs(B) and ginsenosides desorbed from MWCNTs(C)

Fig.2 Adsorption capacity of MWCNTs to the 8 ginsenosides

Fig.3 Recovery of the 8 ginsenosides separated by MWCNTsa. Rd; b. Rc; c. Ro; d. Rb2; e. Rb1; f. 20(S)-Rf;g. Rg1; h. Re.

Fig.4 Recovery of the 8 ginsenosides desorbed by different kinds of solventa. 40% Ethanol; b. 70% ethanol; c. acetonitrile;d. acetone; e. n-BuOH; f. 95% ethanol.

Fig.5 Time courses of adsorption(A) and desorption(B) of the 8 ginsenosides on MWCNTs

Fig.6 Recovery of the 8 ginsenosides desorbed by different volumes of solvent

Fig.7 Adsorption capacity of D100 macroporous resin to the 8 ginsenosides

Fig.8 Time courses of adsorption(A) and desorption(B) of the 8 ginsenosides on D100 macroporous resin

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