Analysis of DNA Fragments and PCR Products from Oncogene Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Abstract

Abstract: Capillary electrophoresis-laser induced fluorescence detection system is applied to detect DNAfragments and PCRproducts from oncogene using hydroxypropylmethylcellulose and no-cross-linked polyacrylamide as sieving media.The effects of sieving medium concentration,electrolyte concentration and intercalating agent concentration on separation of DNAfragments have been investigated in detail.The relationship between peak area and concentration of DNAfragments in pressure injection has been compared with that of electrokinetic injection.

Key words: Capillary electrophoresis, Laser-induced fluorescence, No-gel sieving, DNA, Oncogene

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REN Ji-Cun, DENG Xi-Yun, CAO Ya, YAO Kai-Tai. Analysis of DNA Fragments and PCR Products from Oncogene Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection[J]. Chem. J. Chinese Universities, 1996, 17(3): 362.

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Analysis of DNA Fragments and PCR Products from Oncogene Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

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