Studies on the Coupling Technique Between High PerformanceCapillary Electrophoresis and Charge-Coupled Device Detector(Ⅲ)──Quantitative Calculation Methods

Abstract

Abstract: This paper reports two calculation methods,namely,sum peak height and peakvolume,for quantitative calculation of multi- wavelength electropherogram.The three-di-mensional electropherogram,on which the quanlitative messages are obtained,is translatedinto a two-dimensional electropherogram by the use of such data processing procedures assummation of data in fluorescence spectra and so on,and the quantitative calculation is per-formed. The sensitivity of detection,signal to noise ratio, accuracy and precision of calculation result are therefore enhanced.

Key words: Capillary electrophoresis, Charge-coupled device detector, Quantitative calcula-tion

TrendMD:

XIONG Shao-Xiang, LI Jian-Jun, CNENG Jie-Ke. Studies on the Coupling Technique Between High PerformanceCapillary Electrophoresis and Charge-Coupled Device Detector(Ⅲ)──Quantitative Calculation Methods[J]. Chem. J. Chinese Universities, 1996, 17(1): 38.

[1]	QIN Gaizhao, TANG Minghua, LAI Yalin, YUAN Liming. Chiral Metal-organic Cage MOC-PA as a Chiral Stationary Phase for Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220417.
[2]	HU Xueyi, CHEN Miaomiao, FANG Yun, FENG Ruiqin, HAN Huihui. Investigation on Pseudo-polyanions of Cationic Cellulose-Sodium Dodecylbenzenesulfonate^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1464.
[3]	ZOU Haimin, ZHOU Chen, SUN Chengjun, LI Yongxin, YANG Xiaosong, WEN Jun, ZENG Hongyan. Simultaneous Determination of 7 Components in Functional Food for Anti-hangover and Hepatoprotection by Capillary Electrophoresis^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1276.
[4]	HU Can, CHEN Yi. Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1681.
[5]	WEI Yu, CHEN Yufu, ZHOU Qiang, YUAN Qiuyue, TAN Fengyu, XIE Tianyao. Enantioseparation of Underivatized D,L-Serine in Biological Matrices by Capillary Electrophoresis with Contactless Conductivity Detection^† [J]. Chem. J. Chinese Universities, 2014, 35(7): 1409.
[6]	YANG Jiao-Mei, REN Jie, LIU Hui-Qing, WANG Yong-Le, XU Zhong-Qi. Separation and Detection of Neurotransmitter-related Substances in Urine Sample by Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2013, 34(12): 2699.
[7]	QIU Chang-Gui, WANG Xiao-Yu, GUO Zhen-Peng, SONG Li-Juan, CHEN Yi. Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand [J]. Chem. J. Chinese Universities, 2012, 33(03): 470.
[8]	LI Yu-Rong, CHEN Chang-Bao, ZHOU Jie*. Separation of DNA Fragments with a Broad Range of Molecular Weight by Capillary Electrophoresis with Sieving Matrix of Poly(ethylene oxide) [J]. Chem. J. Chinese Universities, 2011, 32(4): 844.
[9]	XIA Zhi-Ning*, LI Li-Xian, CHEN Hua, XIONG Cai-Qiao, YANG Feng-Qing. Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE [J]. Chem. J. Chinese Universities, 2011, 32(4): 851.
[10]	WANG Xiao-Yu, CHEN Yi*. Online Base Barrage Focusing and Capillary Electrophoresis of Aromatic Amines [J]. Chem. J. Chinese Universities, 2010, 31(8): 1506.
[11]	XU Zhang-Run, LI Na, ZHANG Hui-Dan, FAN Xiao-Feng, FANG Jin. On-chip Temperature Gradient Capillary Electrophoresis with Thermal Resistance Gradient Heating System for DNA Mutation Detection [J]. Chem. J. Chinese Universities, 2010, 31(4): 667.
[12]	XIE Ming-Yi, GUO Zhen-Peng, CHEN Yi*. Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2010, 31(11): 2162.
[13]	QU Ling-Li, LI Yuan-Qian*, ZHENG Bo, HE Cheng-Yan, HE Ling, LI Yong-Xin . Simultaneous Multi-response Optimization and Capillary Electrophoresis with Laser Induced Fluorescence Detector for Rapid Detection of Foodborne Pathogenic Bacteria [J]. Chem. J. Chinese Universities, 2009, 30(6): 1121.
[14]	LIU Yan-Ming^*, TIAN Wei. Sensitive Determination of Drug Alkaloids by Capillary Electrophoresis with Electrochemiluminescence Detection and Its Applications to Analysis of Human Urine [J]. Chem. J. Chinese Universities, 2009, 30(1): 51.
[15]	ZHANG Li-Wei, ZHANG Xin-Xiang*. Interactions Between Fluoroquinolones and Bovine Serum Albumin by Fluorescence Method and Affinity Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2008, 29(4): 694.

Studies on the Coupling Technique Between High PerformanceCapillary Electrophoresis and Charge-Coupled Device Detector(Ⅲ)──Quantitative Calculation Methods

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments