Determination of Urease Activity by Conductometry

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (9): 1377.

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Determination of Urease Activity by Conductometry

ZHU Yuan-Bao, YAN Liu-Shui, HE Shuang-E

Department of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082

Received:1994-09-29 Revised:1995-05-29 Online:1995-09-24 Published:1995-09-24

Abstract

Abstract: A rapid and simple conductometric method has been estabilished for the assay of urease activity.Experimental results showed that the method has the advantages of high sensitivity and wide linear range of 0. 02 to 0. 03U/ mL,and does not need other reagents except substrate.The Michaelis constant and activation energy of the enzymatical reaction were also determined,which are in good agreement with previously reported values.Furthermore,the proposed method had been applied to assay urease content from swordbean and soybean with a RSD within 0.8% and a recovery of 95% to 105%.

Key words: Urease activity, Conductometry, Michaelis constant, Activation energy

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ZHU Yuan-Bao, YAN Liu-Shui, HE Shuang-E. Determination of Urease Activity by Conductometry[J]. Chem. J. Chinese Universities, 1995, 16(9): 1377.

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Determination of Urease Activity by Conductometry

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