Microcalorimetry Applied to the Study of Catalase Reaction

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (6): 924.

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Microcalorimetry Applied to the Study of Catalase Reaction

LIANG Yi¹, WANG Cun-Xin¹, WU Ding-Quan¹, SONG Zhao-Hua¹, QU Song-Sheng¹, ZOU Guo-Lin²

1. Department of Chemistry, Wuhan University, Wuhan, 430072;
2. College of Life Science, Wuhan University, Wuhan, 430072

Received:1994-06-15 Revised:1994-12-03 Online:1995-06-24 Published:1995-06-24

Abstract

Abstract: Microcalorimetry and thermokinetic equations were used to study H₂O₂decomposition by beef liver catalase.The catalase reaction obeyed the Michaelis-Menten kinetics,and at 298.15 K and pH 7.0,the Michaelis constant (K_m),the turnover number and the molar enthalpy(Δ_rH_m)of the reaction were 2.36×10^-2mol/L,1.20×104s^-1and -83.67 kJ·mol^-1,respectively.The later period of catalase reaction was a first order reaction for H₂O₂,=6.31×10⁵L·mol^-1·s^-1and k₁=6.31×10^-5/[E_o]s^-1,respectively.The catalase reaction obeyed Ogura mechanism,and the ternary complex decomposition rate constant was 6.00×10³s^-1.

Key words: Microcalorimetry, Catalase, Michaelis constant, Thermokinetic equation, Reaction mechanism

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LIANG Yi, WANG Cun-Xin, WU Ding-Quan, SONG Zhao-Hua, QU Song-Sheng, ZOU Guo-Lin. Microcalorimetry Applied to the Study of Catalase Reaction[J]. Chem. J. Chinese Universities, 1995, 16(6): 924.

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Microcalorimetry Applied to the Study of Catalase Reaction

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