The Chemical Modification of Tryptophan Residues in Polygalacturonase

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (2): 211.

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The Chemical Modification of Tryptophan Residues in Polygalacturonase

WANG Hong-Mei, LIU Jia-Li, YANG Ping, LIU Lan-Ying

Department of Molecular Biology, Jilin University, Changchun, 130023

Received:1994-02-25 Revised:1994-11-12 Online:1995-02-24 Published:1995-02-24

Abstract

Abstract: The tryptophan residues in polygalacturonase(PG2) had been modified by N-bromosuccinimide(NBS) to study the relationship between the Trp residues and the activity of the enzyme.The results indicate that there are six Trp residues in PG2.Four of them locate in the interior and the other two locate on the surface of the enzyme molecule.One of the Trp residues,which is on the surface of PG2,is essential for the activity of the enzyme and locates at the active site of the enzyme.The spectra of circular dichroism(CD) and fluorescence of PG2 are changed after treatment with NBS.

Key words: Polygalacturonase, Tryptophan, Chemical modification.

TrendMD:

WANG Hong-Mei, LIU Jia-Li, YANG Ping, LIU Lan-Ying. The Chemical Modification of Tryptophan Residues in Polygalacturonase[J]. Chem. J. Chinese Universities, 1995, 16(2): 211.

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The Chemical Modification of Tryptophan Residues in Polygalacturonase

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