Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and1-Butanol in Organic Systems

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (9): 1335.

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Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and₁-Butanol in Organic Systems

XIE Zhi-Dong¹, HE Bing-Lin¹, LU Xian-Yu²

1. Institute of Polymer Chemistry, Nankai University, Tianjin, 300071;
2. Department of Biology, Nankai University

Received:1993-11-22 Revised:1994-03-28 Online:1994-09-24 Published:1994-09-24

Abstract

Abstract: Series of macroporous cross-linked polymers of styrene with divinylbenzene and their functionalization products were synthesized. Effects of degree of cross-linking, amount of pore-generating agent and different aminating agents on immobilization of porcine pancreas lipase(PPL)were studied.The best carrier was selected.Transesterification of methyl butyrate with 1-butanol was catalyzed by the free and immobilized PPL, it was found that the catalytic activity of immobilized PPLin organic systems was two times as high as that of the free PPL.

Key words: Polystyrene, Porcine pancreas lipase, Immobilization, Organic systems, Transesterification

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XIE Zhi-Dong, HE Bing-Lin, LU Xian-Yu. Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and₁-Butanol in Organic Systems[J]. Chem. J. Chinese Universities, 1994, 15(9): 1335.

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Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and₁-Butanol in Organic Systems

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