Biofunctional Electrodes(V)─Immobilization of Glucose Oxidase in α-Cyclodextrin Polymer

Abstract

Abstract: This paper describes a new design of glucose enzyme sensor in which glucose oxidase(GOD)is immobilized in a condensation α-cyclodextrin polymer membrane and a mediator is molecularly included in the cyclodextrin cavity.The FTIRmeasurement of the immobilized enzyme membrane indicated that GODwas attached to the cyclodextrin polymer by covalent bonding.The different GODenzyme electrodes with mediators were prepared and their performances were compared.The GODelectrode with tetrathiafulvalene showed good amperometric response to glucose in test solution, and promised well to be the second generation glucosesensor.

Key words: Enzyme electrodes, Glucose oxidase, Cyclodextrin, Enzyme immobilization, Host-guest complexation

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WU Hui-Huang, WU BaO-Zhang, ZHOU Shao-Min. Biofunctional Electrodes(V)─Immobilization of Glucose Oxidase in α-Cyclodextrin Polymer[J]. Chem. J. Chinese Universities, 1994, 15(7): 1026.

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Biofunctional Electrodes(V)─Immobilization of Glucose Oxidase in α-Cyclodextrin Polymer

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