Abstract: Three types of Nafion/Os(bpy)₃Cl₂ modified electrodes were prepared:(A)acetylene black powder microelectrode.(B) Teflon bonded acetylene black membrane electrode and (C) flat glassy carbon electrode,Experimental results show that the ratios of the redox center loading level for unit apparent electrode surface area are A:B:C= 103:102:1.Besides,it was found to be easier for Athan Band Cto form a thin and relatively uniform coating layer on the real surface,which is benificial to charge transfer in the modified layer,Aglucose oxidase(GOD) electrode was built based on the modified powder microelectrode,which gave an apparent current density 4.6 × 10^-4 A/cm² in 10 mmol/Lglucose solutions,about three orders of magnitude greater than that of an anolog of a flat glassy carbon electrode. The linear region of the former was up to 14 mmol/L, also better than the latter.

Key words: Modified electrode, Enzyme electrode, Porous electrode