Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (5): 667.

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The Study of Retroreflective Laser Beams Interference Phase-Shift Effect

DENG Yan-Zhuo, HE Jin-Lan   

  1. Department of Analysis and Testing Sciences, Wuhan University, Wuhan, 430072
  • Received:1993-10-05 Revised:1993-12-24 Online:1994-05-24 Published:1994-05-24

Abstract: This paper describes a novel optical interference effect, namely retroreflective laser beams interference phase-shift effect. When a focused He-Ne laser beam passes through a cuvette containing liquid sample, the two reflective beams (retroreflective beams)coming from the front and the back surfaces of the cuvette will recombine and generate optical interference effect. The optical-phase-shift between retroreflective beams at the time t depends upon the changes in refractive index of the sample △n. It has been deduced that the relative change of signal intensity at the detector is proportional to △n under the condition of △nl<<λ, where l is the length of the sample within the cuvette and λ is the laser wavelength.The possibility of determining the small changes in the concentration of micro-volume sample was verified experimentally. Its application to the capillary electrophoresis detector was also demonstrated.

Key words: Laser, Refractive index, Interference phase-shift effect, Capillary electrophoresis

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