Studies on a-Amylase from Bacillus Subtilis 86315(Ⅰ)--The Thermal Stability of a-Amylase

Abstract

Abstract: The thermal stability and the fluorescence spectra of α-amylase from bacillus subtilis 86315 are studied in this paper. The enzyme is stable below 50℃. Heated at 70℃ for 10 min the enzyme loses its all activity, at the same time, a considerable change of its fluorescence spectra was observed. The α-amylase in the solution including 0. 02 mol/L CaCl₂and 0. 02 mol/L NaCl retains its activity on heating at 70℃ for 10 min, and its fluorescence spectrum is close to that of the native enzyme. The experimental results show that a proper ratio of CaClz to NaCl could stabilize the conformation of a-amylase, thus raising its thermal stability greatly.

Key words: Bacillus subtilis86315, α-Amylase, Thermal stability, Fluorescence spectra

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Shi Yong-chang, Jiang Yong-ming, Sui De-xin, Wu Yan . Studies on a-Amylase from Bacillus Subtilis 86315(Ⅰ)--The Thermal Stability of a-Amylase[J]. Chem. J. Chinese Universities, 1991, 12(5): 703.

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Studies on a-Amylase from Bacillus Subtilis 86315(Ⅰ)--The Thermal Stability of a-Amylase

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