Chem. J. Chinese Universities ›› 2018, Vol. 39 ›› Issue (11): 2445.doi: 10.7503/cjcu20180168
• Organic Chemistry • Previous Articles Next Articles
DU Shanshan1, LI Yang1, GUO Lei2, LI Pengyu1, CHAI Zhilong1, WANG Tao2, QUAN Dongqin2, HE Junlin2,*()
Received:
2018-03-04
Online:
2018-11-10
Published:
2018-10-10
Contact:
HE Junlin
E-mail:hejunlin@bmi.ac.cn
Supported by:
CLC Number:
TrendMD:
DU Shanshan, LI Yang, GUO Lei, LI Pengyu, CHAI Zhilong, WANG Tao, QUAN Dongqin, HE Junlin. Modification of Aptamer TBA with Extra Functional Groups and the Biological Activities†[J]. Chem. J. Chinese Universities, 2018, 39(11): 2445.
Name | Sequences d(5'-3') | MS, m/z(calcd. ) |
---|---|---|
TBA | GGT TGG TGT GGT TGG | 4727.4(4726.0) |
TBA-T3-1 | GG1 TGG TGT GGT TGG | 4813.8(4812.1) |
TBA-T4-1 | GGT1GG TGT GGT TGG | 4814.0(4812.1) |
TBA-T12-1 | GGT TGG TGT GG1 TGG | 4814.3(4812.1) |
TBA-T13-1 | GGT TGG TGT GGT1GG | 4815.4(4812.1) |
TBA-T3-T12-1 | GG1 TGG TGT GG1 TGG | 4899.1(4898.2) |
TBA-T4-T13-1 | GGT1GG TGT GGT 1GG | 4898.9(4898.2) |
Table 1 TBA and its analogues modified with the monomer 1 and the mass spectra data
Name | Sequences d(5'-3') | MS, m/z(calcd. ) |
---|---|---|
TBA | GGT TGG TGT GGT TGG | 4727.4(4726.0) |
TBA-T3-1 | GG1 TGG TGT GGT TGG | 4813.8(4812.1) |
TBA-T4-1 | GGT1GG TGT GGT TGG | 4814.0(4812.1) |
TBA-T12-1 | GGT TGG TGT GG1 TGG | 4814.3(4812.1) |
TBA-T13-1 | GGT TGG TGT GGT1GG | 4815.4(4812.1) |
TBA-T3-T12-1 | GG1 TGG TGT GG1 TGG | 4899.1(4898.2) |
TBA-T4-T13-1 | GGT1GG TGT GGT 1GG | 4898.9(4898.2) |
Name | Tm/℃ | Name | Tm/℃ |
---|---|---|---|
TBA | 35.0 | TBA-T13-1 | 30.6 |
TBA-T3-1 | 36.1 | TBA-T3-T12-1 | 33.7 |
TBA-T4-1 | 30.5 | TBA-T4-T13-1 | 27.4 |
TBA-T12-1 | 34.6 |
Table 2 Thermal stability of TBA and its analogues*
Name | Tm/℃ | Name | Tm/℃ |
---|---|---|---|
TBA | 35.0 | TBA-T13-1 | 30.6 |
TBA-T3-1 | 36.1 | TBA-T3-T12-1 | 33.7 |
TBA-T4-1 | 30.5 | TBA-T4-T13-1 | 27.4 |
TBA-T12-1 | 34.6 |
Fig. 1 CD spectra of TBA and its analogue(A), (B) in PBS(138 mmol/L NaCl+27 mmol/L KCl+10 mmol/L Na2HPO4+1.76 mmol/L KH2PO4, pH=7.4); (C)—(I) CD spectra of TBA and its analogue in the presence of thrombin(6 U, with incubation time of 5 min(labeled with a) and overnight(labeled with b) in PBS. (A) a. TBA; b. TBA-T3-1; c. TBA-T12-1; d. TBA-T3-T12-1; (B) a. TBA; b. TBA-T4-1; c. TBA-T13-1; d. TBA-T4-T13-1; (C) a. TBA; b. TBA-a; c. TBA-b; (D) a. TBA-T3-1; b. TBA-T3-1-a; c. TBA-T3-1-b; (E) a. TBA-T4-1; b. TBA-T4-1-a; c. TBA-T4-1-b; (F) a. TBA-T12-1; b. TBA-T12-1-a; c. TBA-T12-1-b; (G) a. TBA-T13-1; b. TBA-T13-1-a; c. TBA-T13-1-b; (H) a. TBA-T3-T12-1; b. TBA-T3-T12-1-a; c. TBA-T3-T12-1-b; (I) a. TBA-T4-T13-1; b. TBA-T4-T13-1-a; c. TBA-T4-T13-1-b.
Name | Clotting time/s | Anticoagulant effect/s | Name | Clotting time/s | Anticoagulant effect/s |
---|---|---|---|---|---|
Control | 20.1±0.2 | | TBA-T12-1 | 31.2±0.6 | 11.1 |
TBA | 28.4±0.1 | 8.3 | TBA-T13-1 | 24.4±0.1 | 4.3 |
TBA-T3-1 | 30.6±0.4 | 10.5 | TBA-T3-T12-1 | 27.6±0.6 | 7.5 |
TBA-T4-1 | 23.6±0.5 | 3.5 | TBA-T4-T13-1 | 24.3±0.2 | 4.2 |
Table 3 Anti-anticoagulant effect of TBA and its analogues
Name | Clotting time/s | Anticoagulant effect/s | Name | Clotting time/s | Anticoagulant effect/s |
---|---|---|---|---|---|
Control | 20.1±0.2 | | TBA-T12-1 | 31.2±0.6 | 11.1 |
TBA | 28.4±0.1 | 8.3 | TBA-T13-1 | 24.4±0.1 | 4.3 |
TBA-T3-1 | 30.6±0.4 | 10.5 | TBA-T3-T12-1 | 27.6±0.6 | 7.5 |
TBA-T4-1 | 23.6±0.5 | 3.5 | TBA-T4-T13-1 | 24.3±0.2 | 4.2 |
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