Studies on Fluorescence Reaction Catalyzed by Mimetic Enzyme of Peroxidase--System of MnTPPS4-HVA-H2O2

Abstract

Abstract: A fluorescence reaction of homovanillic acid with hydrogen peroxide catalyzed by mimetic enzyme of peroxidase (manganese tetrakis(sulfophenyl) porphyrin) was investigated. Ahigh sensitive fluorometric method for the determination of hydrogen peroxide and glucose has been developed. The sensitivities of the determination of hydrogen peroxide and glucose are 8.5×10^-8mol/Lfor hydrogen peroxide and 0.5μg·mL^-1for glucose, respectively. The recommended method was applied to determine hydrogen peroxide in snow and glucose in human serum and satisfactory results were obtained.

Key words: Mimetic enzyme of peroxidase, Determination of hydrogen peroxide by fluorescence method, Glucose

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Ci Yunxiang, Wang Fang . Studies on Fluorescence Reaction Catalyzed by Mimetic Enzyme of Peroxidase--System of MnTPPS₄-HVA-H₂O₂[J]. Chem. J. Chinese Universities, 1989, 10(3): 229.

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Studies on Fluorescence Reaction Catalyzed by Mimetic Enzyme of Peroxidase--System of MnTPPS₄-HVA-H₂O₂

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