Mesoporous Organosilicon Hollow Nanospheres Immobilized Uricase for the Determination of Uric Acid

doi:10.7503/cjcu20240346

Abstract

Abstract:

Mesoporous organosilicon hollow nanospheres（MONs） were synthesized through a one-step growth- induced etching method， and then uricase was successfully immobilized onto MONs， forming MONs-uircase. Comprehensive characterizations of MONs and MONs-uricase were performed using scanning electron microscopy （SEM）， transmission electron microscopy（TEM）， infrared spectroscopy， N₂ adsorption-desorption measurements， and thermogravimetric analysis（TGA）. The results indicated that uricase was successfully immobilized within the mesoporous structure of MONs， with an immobilization efficiency exceeding 90%. MONs possessed excellent adsorption properties due to their mesoporous channels and hollow structures. Compared to free uricase， MONs-uricase exhibited enhanced thermal and pH stability. In the detection of serum uric acid（UA）， MONs-uricase demonstrated a good linear relationship within the UA concentration range of 0.01—1.00 mg/mL， along with high specificity. MONs-uricase could be readily recovered through centrifugation， and the recovered MONs-uricase maintained high enzymatic activity after multiple cycles of use， retaining 50% enzymatic activity even after 20 cycles， indicating its excellent reusability. MONs offered promising application prospects in the immobilization and recycling of biological enzymes.

Key words: Mesoporous organosilicon hollow nanospheres, Immobilization of uricase, Determination of uric acid

CLC Number:

O613

TrendMD:

JI Heming, ZHANG Yuhang, MIAO Tingting, WANG Yiqian, YU Xiaorui, WANG Chunyan, WANG Runwei. Mesoporous Organosilicon Hollow Nanospheres Immobilized Uricase for the Determination of Uric Acid[J]. Chem. J. Chinese Universities, 2024, 45(12): 20240346.

Figures/Tables 10

References 32

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Sample	Serum sample	UA standard solution	MONs⁃uricase	MONs	Glycine buffer	Deionized water
Test	0.4 mL	0.1 mL	3.0 mg	0 mg	2.0 mL	0.5 mL
Control	0.4 mL	0 mL	3.0 mg	0 mg	2.1 mL	0.5 mL
Standard	0 mL	0.1 mL	3.0 mg	0 mg	2.4 mL	0.5 mL
Blank	0 mL	0 mL	0 mg	3.0 mg	2.5 mL	0.5 mL

Sample	Serum sample	UA standard solution	MONs⁃uricase	MONs	Glycine buffer	Deionized water
Test	0.4 mL	0.1 mL	3.0 mg	0 mg	2.0 mL	0.5 mL
Control	0.4 mL	0 mL	3.0 mg	0 mg	2.1 mL	0.5 mL
Standard	0 mL	0.1 mL	3.0 mg	0 mg	2.4 mL	0.5 mL
Blank	0 mL	0 mL	0 mg	3.0 mg	2.5 mL	0.5 mL

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