Preparation of Fe3O4 Nanoparticles and Lentiviral Vector Infected EPCs for the Improvement of Angiogenesis and MRI Tracing†

doi:10.7503/cjcu20160180

Abstract

Abstract:

Fe₃O₄ nanoparticles(NPs) modified with sodium citrate were prepared via chemical coprecipitation method and fetal bovine serum(FBS) could improve the dispersibility of Fe₃O₄ NPs in FBS solution(volume fraction≤5%). At the temperature of 300 K, the saturation magnetization intensity of NPs reached 74.86×10^-3 A·m²/g(74.86 emu/g); MRI T2 imaging of endothelial progenitor cells(EPCs) became better after being infected by 75 μg/mL of Fe₃O₄NPs and lentiviral vectors(LV); furthermore, EPCs could stably over express VEGF target gene. EPCs co-infected with Fe₃O₄ NPs and LV, after being injected into caudal vein of focal cerebral ischemia rats could effectively promote angiogenesis. These results indicated the modified EPCs possessed both MRI tracing and angiogenesis, and therefore had potential application value in clinical therapy of cerebral ischemia.

Key words: Fe3O4 nanoparticles, Endothelial progenitor cell, Lentiviral vector, Vascular endothelial growth factor, Magnetic resonance imaging

CLC Number:

O631
O647

TrendMD:

WANG Juan, ZHOU Bing, WANG Xinxin, WU Xiujuan, LI Ting, ZHANG Wenke, LIU Kangding. Preparation of Fe₃O₄ Nanoparticles and Lentiviral Vector Infected EPCs for the Improvement of Angiogenesis and MRI Tracing^†[J]. Chem. J. Chinese Universities, 2016, 37(6): 1148.

Figures/Tables 9

Fig.1 TEM(A) and HRTEM(B) images of Fe3O4 NPs

Fig.2 Particle size distribution histogram(A) and electron diffraction pattern(B) of Fe3O4 NPs

Fig.3 FTIR spectrum of sodium citrate modified Fe3O4 NPs

Fig.4 Particle size distribution of Fe3O4 NPs from dynamic light scattering analysis(A), zeta potential distribution of Fe3O4 NPs coated with sodium citrate in FBS solution at pH=7.0(B) and magnetic hysteresis curve of Fe3O4 NPs measured at 300 K(C)

Fig.5 Photographs of Fe3O4 NPs in 5%FBS solution(A) and water(B) after 6 months

Fig.6 MRI T2 imaging of EPCs infected by LV and various concentrations of Fe3O4 NPs

Fig.7 Images of EPCs infected by LV and Fe3O4 NPs under white light(A) and UV light(B)

Fig.8 Western blotting results(A) and protein relative expression of VEGF(B) in different groupsa. LV; b. LV(VEGF); c. LV(VEGF)-Fe3O4 NPs.

Fig.9 Immunohistochemistry of CD34 in the brain of MCAO rats in EPCs-VEGF-Fe3O4 NPs treatment group(A), EPCs-VEGF treatment group(B) and normal saline treatment group(C)

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Preparation of Fe₃O₄ Nanoparticles and Lentiviral Vector Infected EPCs for the Improvement of Angiogenesis and MRI Tracing^†

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