Escherichia Coli DNA Biosensor Using Terephthalic Acid as Arm Linker

doi:10.7503/cjcu20130075

Abstract

Abstract:

Ahomogeneous CdS-PVA(polyvinyl alcohol) nanocomposite dispersion was obtained by mixing hollow CdS nanospheres in PVA solution under ultrasonic condition. Then the dispersion was dropped on a glassy carbon electrode for dryness to obtain the CdS-PVA modified electrode. Further, a novel DNA biosensor for Escherichia coli was fabricated by covalent immobilization of Escherichia coli gene related oligonucleotides on the modified electrode using terephthalic acid as the arm linker. The analytical performance of the biosensor was investigated by electrochemical impedance method, and the results revealed that the constructed biosensor had a wide dynamic range from 1.0×10^-12 mol/L to 1.0×10^-7 mol/L and a low detection limit of 1.3×10^-13 mol/L. The selectivity experiments showed that the DNA biosensor could accurately discriminate the complementary sequence from the single-base mismatched, three-base mismatched and noncomplementay sequences. When the biosensor was applied for the detection of the real sample of Escherichia coli, a satisfying result was obtained.

Key words: DNA biosensor, Hollow CdS nanospheres, Polyvinyl alcohol, Electrochemical impedance spectroscopy

CLC Number:

O657
O626

TrendMD:

WANG Li-Heng, GAO Feng, JIANG Shu-Lian, DING Ying-Tao, ZHENG De-Lun, HUANG Li-Zhang, WANG Qing-Xiang. Escherichia Coli DNA Biosensor Using Terephthalic Acid as Arm Linker[J]. Chem. J. Chinese Universities, 2013, 34(5): 1072.

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Escherichia Coli DNA Biosensor Using Terephthalic Acid as Arm Linker

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