Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (7): 1612.doi: 10.7503/cjcu20121164

• Analytical Chemistry • Previous Articles     Next Articles

Electrochemical Immunosensor Based on AuNPs/PDDA-GO for Detection of SirT1

BI Wen-Ji, LU Dan-Qin, FU Ying, HUANG Qi-Lin, XU Zhi-Ai, ZHANG Wen   

  1. Department of Chemistry, East China Normal University, Shanghai 200241, China
  • Received:2012-12-27 Online:2013-07-10 Published:2013-06-21
  • Contact: wen zhang E-mail:wzhang@chem.ecnu.edu.cn;zaxu@chem.ecnu.edu.cn

Abstract:

AuNPs/PDDA-GO nanocomposite was produced by combining poly(diallyldimethylammonium chloride)(PDDA) functionalized graphene oxide nanosheets(PDDA-GO) and gold nanoparticles(AuNPs) through self-assembly method, which offered an efficient platform for antibody immobilization with good biocompatibility. A new type of electrochemical immunosensor based on AuNPs/PDDA-GO nanocomposite for the detection of SirT1 was then developed. The immunosensor construction consisted of the immobilization of the hybrid architecture, the immunoreaction of target antigen SirT1 and capture antibody(Ab1), followed by the binding of HRP-Ab2. The designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range for SirT1 from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.029 ng/mL. Overall, this developed strategy is proved as a simple, cost-effective method, and can be easily extended to other protein analysis.

Key words: AuNPs/PDDA-GO nanocomposite, SirT1, Electrochemical immunosensor

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