Screening the Inhibitors of Tau Protein Kinase 2 by Rapid Resolution Liquid Chromatogrophy-Mass Spectrometry

doi:10.7503/cjcu20120786

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (5): 1111.doi: 10.7503/cjcu20120786

• Analytical Chemistry • Previous Articles Next Articles

Screening the Inhibitors of Tau Protein Kinase 2 by Rapid Resolution Liquid Chromatogrophy-Mass Spectrometry

CHEN Ning^1,2, SUN Yi³, YUE Hao³, LIU Shu-Ying^1,3

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China

Received:2012-08-28 Online:2013-05-10 Published:2013-05-10

Abstract

Abstract:

A method based on rapid resolution liquid chromatogrophy-mass spectrometry was developed for screening the inhibitor of Tau protein kinase 2(TPK2) from natural products. Tau protein kinase 2(TPK2) is a serine/threonine kinase, which is reported to play an important role in the pathogenesis of Alzheimer's disease. The excellent substrate, histone-based peptide PKTPKKAKKL was used in the study. The product(PKpTPKKAKKL) and the internal standard(PKpTPKKAKKV) were separated by RRLC on a C₁₈ column within 4 min, and quantified by electrospray ionization mass spectrometry(ESI-MS). An online desalting procedure was carried out in the experiment without sample pretreatment prior to analysis. Moreover, the enzymatic reaction conditions including pH value, temperature, reaction time, the concentration of kinase were optimized.

Key words: Alzheimer's disease, Tau protein kinase, Rapid resolution liquid chromatography/mass spectrometry

CLC Number:

O657

TrendMD:

CHEN Ning, SUN Yi, YUE Hao, LIU Shu-Ying. Screening the Inhibitors of Tau Protein Kinase 2 by Rapid Resolution Liquid Chromatogrophy-Mass Spectrometry[J]. Chem. J. Chinese Universities, 2013, 34(5): 1111.

[1]	WANG Fangyuan, ZHANG Fenxian, LI Yi, GAO Jianhua, NIU Yanbing, SHEN Shaofei. Fabrication of Bionic Leaf Model and Its Application in Agarose Microfluidic Chip [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220445.
[2]	ZHANG Liling, LIU Liu, ZHENG Mingqiu, FANG Wenkai, LIU Da, TANG Hongwu. Dual Signal Detection of HPV16 DNA by CRISPR/Cas12a Biosensing System Based on Upconversion Luminescent Resonance Energy Transfer [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220412.
[3]	QIN Gaizhao, TANG Minghua, LAI Yalin, YUAN Liming. Chiral Metal-organic Cage MOC-PA as a Chiral Stationary Phase for Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220417.
[4]	XU Ruotao, WANG Qiang, WANG Weiyu, BAO Qingjia, ZHANG Zhi, LIU Zhaoyang, XU Jun, DENG Feng. In situ NMR Imaging of Solvent Infiltration on γ-Al2O3 particles [J]. Chem. J. Chinese Universities, 0, (): 20220587.
[5]	ZHAO Xueqi, ZHAO Yue, XUE Jing, BAI Min, CHEN Feng, SUN Ying, SONG Daqian, ZHAO Yongxi. Nucleic Acids-Encoded Amplification for Single-cell Imaging [J]. Chem. J. Chinese Universities, 0, (): 20220572.
[6]	ZHAO Hengzhi, YU Fangzhi, LI Xiangfei, LI Lele. Advances in Biosensing and imaging Based on the Integration of DNA and UCNPs [J]. Chem. J. Chinese Universities, 0, (): 20220626.
[7]	WANG Shiqi, LUO Bowen, YU Jicheng, GU Zhen. Near-Infrared-Ⅱ Fluorescence Imaging for Tumor Diagnosis and Therapy [J]. Chem. J. Chinese Universities, 0, (): 20220577.
[8]	SONG Lu, ZHANG Shuyang, WANG Lihua, ZUO Xiaolei, LI Min. High-throughput Biological Microarrays Based on Framework Nucleic Acids [J]. Chem. J. Chinese Universities, 0, (): 20220563.
[9]	WANG Wei, ZOU Bingchen, HOU Jie, ZHOU Wanli, LUO Jianping, WANG Kangli, JIANG Kai. In⁃situ Analysis of Interfacial Reaction Process Inside Li-Ga Liquid Metal Battery [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220261.
[10]	CHEN Jialu, HUANG Shuo. Application of Nanopore Sequencing Technology in the Detection of Nucleic Acid Modifications [J]. Chem. J. Chinese Universities, 2022, 43(Album-4): 20220333.
[11]	WANG Di, ZHONG Keli, TANG Lijun, HOU Shuhua, LYU Chunxin. Synthesis of Schiff-based Covalent Organic Framework and Its Recognition of I ^‒ [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220115.
[12]	LIU Miao, LIU Ruibo, LIU Badi, QIAN Ying. Synthesis， Two-photon Fluorescence Imaging and Photodynamic Therapy of Lysosome-targeted Indole-BODIPY Photosensitizer [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220326.
[13]	LI Ao, LI Lingxuan, ZUO Cuicui, CHEN Chuankai, FAN Yifan, BU Yifan, LIN Hongyu, GAO Jinhao. A Boronate-Based 19F NMR/MRI Molecular Probe for Activatable Deep-Tissue Imaging of Reactive Oxygen Species [J]. Chem. J. Chinese Universities, 0, (): 20220545.
[14]	YANG Yanling, YE Deju. Recent Advance in the Development of Molecular Probes for Targeting Carbonic Anhydrases [J]. Chem. J. Chinese Universities, 0, (): 20220557.
[15]	QIN Wenjie, HUANG Yizhuo, LUO Xiao, QIAN Xuhong, YANG Youjun. Probe Design Strategies for Cy7-type Cyanine Dyes [J]. Chem. J. Chinese Universities, 0, (): 20220567.

Screening the Inhibitors of Tau Protein Kinase 2 by Rapid Resolution Liquid Chromatogrophy-Mass Spectrometry

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments