Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (11): 2397.doi: 10.7503/cjcu20120347

• Analytical Chemistry • Previous Articles     Next Articles

cDNA Microarray for the Study of Compatibility Mechanism of Multi-component Chinese Medicine New Shuanglong Formula

LÜ Lin-Xing1, FAN Xue-Mei2, LIANG Qiong-Lin2, WANG Wen-Kai1, WANG Yi-Ming1,2, LUO Guo-An1,2   

  1. 1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;
    2. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2012-04-13 Online:2012-11-10 Published:2012-10-15

Abstract:

The pharmacological mechanism of New Shuanglong Formula with its two effective components total ginsenoside(TGS) and total salvianolic acid(TSA) on acute myocardial infarction(AMI) rats was compared by cDNA microarray. The model was induced by ligation of the left anterior descending artery, and significant genes were screened(Ratio>2 or <0.5) when AMI rats were treated before and after with New Shuanglong Formula and components by genome-wide expression profiles. Through comprehensive analysis of significant genes of four groups, the similarities and differences of effect between New Shuanglong Formula and components were analyzed and the synergy of compound compatibility at molecular control level was discussed; meanwhile, quantitative real time reverse transcription-polymerase chain reaction(RT-PCR) was used to analyse Cacna1d gene(associated with Calcium ion transport)and Flnb gene(associated with Vascular repair) which both were directly related to the pharmacological effects. The analysis of significant genes showed the effect on gene expression of AMI rats was different from New Shuanglong Formula to components, the compound appeared to play a better effect than components. Through clustering analysis of different genes and the comparison of regulation effects by drugs, the compound appeared to play a better effect than componects. Pathway analysis indicates the affects of New Shuanglong Formula and components are mainly through regulating calcium signaling pathway, MAPK signaling pathway and so on, and the compound involved more pathways; common target genes which are regulated by compound and components were screened, although their expression patterns are not exactly the same, but the compound appear to play a better effect. The results of this study have illuminated the advantage of New Shuanglong Formula from the perspective of molecular control.

Key words: Gene expression profile, Real time reverse transcription-polymerase chain reaction(RT-PCR), Compatibility of Traditional Chinese Medicine(TCM), New Shuanglong Formula, Acute Myocardial infarction

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