高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (7): 974.

• 论文 • 上一篇    下一篇

L-天冬氨酸微生物电极的研制

王氢, 邓家祺   

  1. 复旦大学化学系, 上海, 200433
  • 收稿日期:1993-07-05 修回日期:1993-12-31 出版日期:1994-07-24 发布日期:1994-07-24
  • 通讯作者: 邓家棋.
  • 作者简介:王氢,男,27岁,博士研究生.
  • 基金资助:

    国家自然科学基金;中国科学院长春应用化学研究所电分析化学开放实验室资助

A Microbial Electrode for L-Aspartate

WANG Qing, DENG Jia-Qi   

  1. Department of Chemistry, Fudan University, Shanghai, 200433
  • Received:1993-07-05 Revised:1993-12-31 Online:1994-07-24 Published:1994-07-24

摘要: 本文以大肠杆菌为天冬氨酸脱氨反应的酶源,采用氨气敏电极为基础电极研制成L-天冬氨酸微生物电极。对细菌培养条件、固定方法及电极工作条件等进行了研究,测定L-天冬氨酸E~1gC曲线斜率为55mV,线性范围为6.0×10-4~8.0×10-3mol/L,寿命可达10天。

关键词: L-天冬氨酸, 微生物电极, 生物传感器

Abstract: An electrode for the determination of L-Aspartate was prepared.Escherichia coli was used as the biocatalyst and an ammonia gas electrode was used as the base electrode.The effects of the culture medium, immobilized methods of the bacteria and the response characteristics of the electrode were studied.The slope of the L-Aspartate response curve E~-IgCis 55 mV/decade, linear range is 6.0×10-4-8.0×10-3mol/Land life time is 10days.

Key words: L-Aspartate, Miocrobial electrode, Biosensor

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