高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (9): 1498.

• 研究快报 • 上一篇    下一篇

DNA分子结构的多态性研究(Ⅲ)——生物大分子探针吖啶橙诱导λ-DNA由线型向环型的转变

冯喜增, 林璋, 马金, 王琛, 白春礼   

  1. 中国科学院化学研究所, 北京, 100080
  • 收稿日期:1998-04-28 出版日期:1998-09-24 发布日期:1998-09-24
  • 通讯作者: 白春礼
  • 作者简介:冯喜增,男,36岁,博士后.
  • 基金资助:

    中国科学院"九五"重大科研项目及中国博士后科学基金资助课题.

The Investigation of the Various Structures of DNA Molecules(Ⅲ) The Coil toroid Transition of λ-DNA Induced by the Biomolecular Probe AO

FENG Xi-Zeng, LIN Zhang, MA Jin, WANG Chen, BAI Chun-Li   

  1. Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080
  • Received:1998-04-28 Online:1998-09-24 Published:1998-09-24

关键词: &lambda, -DNA, 吖啶橙, 扫描电子显微镜, 原子力显微镜

Abstract: The helix and structure transition of λ-DNAin AOsolutions were investigated by SEMand AFMmethods. Using SEM, we observed the turn and globular as well as toroidal condensates of λ-DNAinduced by AO. The fine structures of these condensates were further observed by using AFM. Our results reveal that the mechanism of the coil-toroid transition of λ-DNAresulted from the changed environment is related to the shrinking and collapsing process as well as the self-assembling process of these condensates.

Key words: λ-DNA, AO, Sanning electron microscopy, Atomic force microscopy

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