高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1401-1406.doi: 10.3969/j.issn.0251-0790.2012.07.007

• 分析化学 • 上一篇    下一篇

单分子力谱研究活细胞上多药耐药相关蛋白1的表达

王青, 孙小兰, 羊小海, 王柯敏, 吴春玲, 陈桐   

  1. 湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院, 生物纳米与分子工程湖南省重点实验室, 长沙 410082
  • 收稿日期:2011-11-21 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 王柯敏, 男, 博士, 教授, 博士生导师, 主要从事纳米及分子水平上的生物分析化学及纳米生物研究. E-mail: kmwang@hnu.edu.cn E-mail:kmwang@hnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 90606003, 20805012)、 国家"九七三"计划项目(批准号: 2011CB911002)、 科技部国际合作重大项目(批准号: 2010DFB30300)、 教育部"新世纪优秀人才支持计划"(批准号: NCET-09-0338)和湖南省高等学校青年骨干教师培养对象基金(2009年度)资助.

Investigation of MRP1 Molecules on Cell Membrane Based on Single Molecule Atomic Force Microscopy

WANG Qing, SUN Xiao-Lan, YANG Xiao-Hai, WANG Ke-Min, WU Chun-Ling, CHEN Tong   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2011-11-21 Online:2012-07-10 Published:2012-07-10

摘要: 采用原子力显微镜的单分子力谱(SMFM)技术研究了多药耐药相关蛋白1(MRP1)与其抗体间的相互作用, 并考察了人舌癌细胞系TCA8113经高剂量平阳霉素(BLM)反复间歇诱导前后细胞表面MRP1的表达差异. 实验结果表明, MRP1与其抗体之间存在特异性相互作用力, 当针尖运动速率为2.5 μm/s时, 作用力大小约为(182±35) pN; 而且药物诱导后MRP1在人舌癌细胞上的表达明显增强. 本工作为了解活细胞水平上MRP1的表达提供了新方法, 有助于肿瘤细胞多药耐药性(MDR)的研究.

关键词: 原子力显微镜, 单分子力谱, 多药耐药性, 多药耐药相关蛋白1(MRP1)表达

Abstract: Multidrug resistance(MDR) is one of the main factors in the failure of chemotherapy against tumors, and multidrug resistance-associated protein 1(MRP1) is closely related to the generation of MDR. In this work, the interaction force between MRP1 and anti-MRP1 was measured via single-molecule force microscopy(SMFM), and the expression differences of MRP1 on human tongue cancer cells before and after treatment with high-dose bleomycin(BLM) were investigated. The results show that the interaction force between MRP1 and anti-MRP1 is about (182±35) pN as the loading rate is 2.5 μm/s. The expression of MRP1 on human tongue cancer cell membrane increased obviously after treatment with high-dose BLM. This work may provide a new method for studying the expression of MRP1 at the living cell level and can be helpful for the study of MDR.

Key words: Atomic force microcopy, Single molecule force spectroscopy, Multidrug resistance(MDR), Expression of MRP1

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