高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (9): 2125.doi: 10.7503/cjcu20121137

• 有机化学 • 上一篇    下一篇

两性霉素B与鞘磷脂单分子层的相互作用

乔进京, 孙润广, 郝长春, 杨静, 马秀梓, 王小梅   

  1. 陕西师范大学物理学与信息技术学院, 生物物理与生物医学工程研究室, 西安 710062
  • 收稿日期:2012-12-18 出版日期:2013-09-10 发布日期:2013-08-30
  • 作者简介:孙润广,男,博士,教授,博士生导师,主要从事生物物理学研究.E-mail:sunrunguang@snnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20772077);教育部高等学校博士学科点专项科研基金(批准号:200807180001);陕西省自然科学基础研究计划(批准号:2012JQ1002);中央高校基本科研业务费专项项目(批准号:GK201102029)资助.

Interaction of Amphotericin B/Sphingomyelin Monolayers at the Air/Water Interface

QIAO Jin-Jing, SUN Run-Guang, HAO Chang-Chun, YANG Jing, MA Xiu-Zi, WANG Xiao-Mei   

  1. Laboratory of Biophysics and Biomedical, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China
  • Received:2012-12-18 Online:2013-09-10 Published:2013-08-30

摘要:

选取哺乳动物生物膜中的重要脂质分子鞘磷脂(SM)作为单分子膜的基本组分, 采用Langmuir-Blodgett(LB)膜技术研究了不同比例的两性霉素B/鞘磷脂单层膜的表面压力-平均分子面积(π-A)曲线以及基于π-A曲线的混合性分析, 同时通过原子力显微镜(AFM)研究了其表面形态的变化. 结果表明, 组分间的摩尔比和表面压力对混合单层膜稳定性、混合性以及分子间相互作用具有重要影响.

关键词: 两性霉素B, 单层膜, 鞘磷脂, π-A曲线, 原子力显微镜

Abstract:

In this work, we chose sphingomyelin(SM) as the basic components of the monolayer, which is widespreaded in mammalian cells and takes an important role in biochemical reactions. Different proportion of amphotericin B/sphingomyelin monolayer's surface pressure and mean molecular area(π-A) curve was investigated by the Langmuir-Blodgett(LB) films technology, thermodynamic characteristics were also analyzed based on the π-A curve and the morphological changes of the surface were observed by atomic force microscopy(AFM). The results showed that the compressibility, stability and thermodynamic characteristics of AmB/SM monolayer depended on the mole faction of AmB and the surface pressure obviously. This may be related to the inhibition of Amphotericin B.

Key words: Amphotericin B, Monolayer, Sphingomyelin, π-A Curve, Atomic force microscopy

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