高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (3): 359.

• 论文 • 上一篇    下一篇

酪氨酸酶电流传感器测定低浓度氰化物抑制剂的研究

胡效亚, 冷宗周   

  1. 扬州大学师范学院化学化工系, 扬州 225002
  • 收稿日期:1994-04-09 修回日期:1994-08-16 出版日期:1995-03-24 发布日期:1995-03-24
  • 通讯作者: 胡效亚,男,34岁,副教授
  • 作者简介:胡效亚,男,34岁,副教授
  • 基金资助:

    江苏省教育委员会自然科学基金

Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide

HU Xiao-Ya, LENG Zong-Zhou   

  1. Department of Chemistry and Chemical Engineering, Teachers College, Yangzhou University, Yangzhou 225002
  • Received:1994-04-09 Revised:1994-08-16 Online:1995-03-24 Published:1995-03-24

PDF (PC)

被引次数

9

摘要： 用预活化聚酰胺膜固定酪氨酸酶,制得性能良好的酪氨酸酶生物传感器,以此测定CN-抑制剂的含量。在磷酸盐缓冲溶液中,以苯酚作底物,浓度范围为5×10-6～4×10-5mol/L,当苯酚浓度一定时,还原电流(-0.200V,vs,SCE)的下降值与CN-浓度的平方根成线性关系,检测范围为5×10-7～2×10-4mol/L,有良好的选择性和重现性。

关键词: 电极, 生物传感器, 酶, 酪氨酸酶, 氰化物

Abstract: Cyandide inhibitor of tyrosinase was determied with biosensor of tyrosinase by using tyrosinase immobilized on a preactivated polyaminde membrane.Phenol with the range of concentration 5×10-6～4×10-5mol/Lwas chosen as substrate in phosphate buffer.The de-crease of substrate response have linear relation with square root of concentration of CN-with detection range of 5×10-7～2×10-4mol/L. The fine selectivity and reproducibility were obtained.

Key words: Electrode, Biosensor, Enzyme, Tyrosinase, Cyanide

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