高等学校化学学报

高等学校化学学报

• 研究简报 • 上一篇    下一篇

[Ru(phen)2dppz]2+二聚及对DNA键合性质的影响

聂晶, 韩美娇, 王科志   

  1. 北京师范大学化学学院, 北京 100875
  • 收稿日期:2007-04-23 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 王科志

Effects of the Dimerization of [Ru(Phen)2dppz]2+ on the Calf Thymus Binding Properties

NIE Jing, HAN Mei-Jiao, WANG Ke-Zhi*   

  1. College of Chemistry, Beijing Normal University, Beijing 100875, China
  • Received:2007-04-23 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
  • Contact: WANG Ke-Zhi

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摘要： 研究发现, [Ru(phen)2dppz]2+表现出非常强的自聚合倾向, 并显著影响DNA的键合性质, 有关方面的研究应引起科研工作者的足够重视.

关键词: 钌, 邻菲啰啉, DNA, 光开关, 二聚

Abstract: The dimerization of a well-known DNA light-switch [Ru(phen)2dppz]2+(phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine), and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCl(pH=7.1). The results show that the complex has a strong tendency to dimerize with a dimerization constant of (1.8±0.3)×106 L/mol at the same order of magnitude as the DNA binding constant of (6.0±1.2)×106 L/mol for the Ru(Ⅱ) complex dimer, but one order of magnitude less than (4.9±1.5)×107 L/mol for the Ru(Ⅱ) complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.

Key words: Ruthenium, Phenanthroline, DNA, Light switch, Dimerization

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