高等学校化学学报

高等学校化学学报

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锰(Ⅲ)Corrole配合物催化DNA氧化断裂

刘海洋1,2, 刘兰英1, 张雷3, 应晓4, 王湘利1, 江焕峰1, 张启光2   

    1. 华南理工大学化学系, 广州 510641;
    2. 香港科技大学化学系, 香港;
    3. 华南理工大学制药工程系,
    4. 应用物理系, 广州 510641
  • 收稿日期:2007-05-08 修回日期:1900-01-01 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 刘海洋

Oxidative DNA Cleavage Catalyzed by Mn(Ⅲ) Corroles

LIU Hai-Yang1,2*, LIU Lan-Ying1, ZHANG Lei3, YING Xiao4, WANG Xiang-Li1, JIANG Huan-Feng1, CHANG Chi-Kwong2*   

    1. Department of Chemistry, South China University of Technology, Guangzou 510641, China;
    2. Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China;
    3. Department of Pharmaceutical Engineering,
    4. Department of Applied Physics, South China University of Technology, Guangzou 510641, China
  • Received:2007-05-08 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
  • Contact: LIU Hai-Yang

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被引次数

40

摘要： 报道了这类单羟基Corrole锰(Ⅲ)配合物对DNA的催化氧化断裂作用.

关键词: 锰, Corrole, DNA, 氧化断裂

Abstract: Several Mn(Ⅲ) 10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-, meta- and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2. The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0, 4.0×10-2 mol/L Tris, 1.0×10-3 mol/L EDTA), 10 μL 1.0×10-5 mol/L Mn(Ⅲ) corrole in DMSO and 10 μL 0.3% H2O2. After 8 h incubation at room temperature, significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ) corrole showing a better activity as detected by agarose-gel electrophoresis. Plasmid DNA was completely damaged after 12 h incubation. These observations show Mn(Ⅲ) corrole can be used as an artificial model of nuclease.

Key words: Manganese, Corrole, DNA, Oxidative cleavage

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