高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (5): 687.

• 研究快报 • 上一篇    下一篇

牛红细胞铜锌超氧化物歧化酶(BESOD)在汞电极表面吸附行为的研究

钱雯, 孟庆国, 罗勤慧, 沈孟长   

  1. 南京大学配位化学研究所, 配位化学国家重点实验室, 南京 2100931
  • 收稿日期:1995-11-29 出版日期:1996-05-24 发布日期:1996-05-24
  • 通讯作者: 罗勤慧
  • 作者简介:钱雯,女,32岁,讲师,博士研究生.
  • 基金资助:

    国家自然科学基金

The Electrochemistry Behaviour of Adsorption of BESOD on Mercury Electrode Surface

QIAN Wen, MENG Qing-Guo, LUO Qin-Hui, SHEN Meng-Chang   

  1. Coordination Chemistry Institute, Coordination Chemistry State Key Laboratory, Nanjing University, Nanjing 210093
  • Received:1995-11-29 Online:1996-05-24 Published:1996-05-24

关键词: 铜锌超氧化物歧化酶, 吸附, 循环伏安, 双阶跃计时电量

Abstract: The electrochemical behaviour of bovine erythrocyte superoxide dismutase(BESOD)adsorbed On the mercury electrode surface was reported for the first time by cyclic voltammetry,direct current polarography and double potential-step chronoculoumetry without using mediator.The contribution of diffusion current predominated over adsorption current when the concentration of BESODwas more than 1.0×10-4mol/L.The experimental adsorption isothermal line was in accordance with that calculated by Langmuir equation.The equilibrium constant of adsorptin was 8.96×104 and Gibbs free energy was -28.2kJ/mol.The molecular area(18.06 nm2) of BESODobtained by the electrochemical method, is in good agreement with that(17.36 nm2determined by X-ray crystal structural analysis.

Key words: Cu, Zn-superoxide dismutase, Adsorption, Cyclic voltammetry.Double potential-step chronocoulometry

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