高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (8): 1218.

• 研究简报 • 上一篇    下一篇

荧光各向异性法研究酸度对四磺基铝酞菁与牛血清白蛋白相互作用的影响

李东辉1, 彭兴跃2, 叶东3, 许金钩3   

  1. 1. 厦门大学抗癌研究中心生物系, 厦门 361005;
    2. 厦门大学抗癌研究中心化学系, 厦门 361005;
    3. 厦门大学抗癌研究中心教育部生命过程与材料分析科学开放研究实验室, 厦门 361005
  • 收稿日期:1998-09-04 出版日期:1999-08-24 发布日期:1999-08-24
  • 通讯作者: 许金钩
  • 作者简介:李东辉,男,31岁,助理研究员,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29775021)资助

Investigation of the Association Between Bovine Serum Albumin(BSA) and Aluminum Tetrasulfonated Phthalocyanine(AlS4Pc) by Fluorescence Anisotropy

LI Dong-Hui1, PENG Xing-Yue2, YE Dong3, XU Jin-Gou3   

  1. 1. Cancer Research Center, Xiamen University, Xiamen 361005;
    2. Department of Biology, Xiamen University, Xiamen 361005;
    3. Department of Chemistry and the Key Laboratory of Analytical Sciences of MOE, Xiamen University, Xiamen 361005
  • Received:1998-09-04 Online:1999-08-24 Published:1999-08-24

PDF (PC)

被引次数

40

关键词: 酞菁, 荧光偏振, 荧光各向异性, 白蛋白

Abstract: Fluorescence anisotropy was employed for the investigation of the association between AlS4Pc and BSA. The average binding number of AlS4Pc to BSAwas calculated and the constants of association reaction occurring in the medium of pH7.0 were shown. The results revealed that the interaction between AlS4Pc and BSAwas strong, which implied that AlS4Pc may be stored and transferred by serum albumin. The merits of the method used were also pointed out.

Key words: Phthalocyanine, Fluorescence polarization, Fluorescence anisotropy, Albumin

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