关键词: 分子印迹聚合物, 三肽, 谷胱甘肽, 高压汞灯, 荧光测定

Abstract: GSH molecularly imprinted polymers were synthesized with UV spot high pressure mercury lamp. This realized the preparation of MIPs in which the template molecules were unstable by means of reducing the time of polymerization from 48 to 2 h at a low temperature. A series of binding experiments in static state was done and the binding performances of MIPs with different molar ratios of templates to monomers was observed by using fluorescence measurement. The character trials which involved the determination of the binding isothermal, the analysis of Scatchard plot and the TLC separation tests were carried out on the MIPs in which the molar ratio of templates to monomers was 1∶8. In these experiments, the MIPs showed comparatively excellent characters. For example, the binding amounts reached 45.4 mg/g and the particularity factors 4.98 were achieved in binding experiments. From Scatchard analysis, it can be known that there were two sorts of binding sites of which the saturated binding amounts were 20.2 and 64.5 mg/g and the equilibrium dissociation constants were 0.435 and 1.48 mmol/L, respectively. The MIPs represented the best separation ability in TLC tests that was attested by the most separation factors of Gly or Glu to GSH on the TLC of MIP, NMIP and silica gel.

Key words: Molecularly imprinted polymer, Tri-peptide, GSH, High pressure Hg lamp, Fluorescence determination