高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (6): 870.

• 研究快报 • 上一篇    下一篇

雌二醇的水凝胶毛细管电泳免疫分析

苏萍, 王永成, 张新祥, 孙玲, 常文保   

  1. 北京大学化学与分子工程学院, 北京 100871
  • 收稿日期:2000-01-06 出版日期:2000-06-24 发布日期:2000-06-24
  • 通讯作者: 张新祥(1966年出生),男,博士,副教授,主要从事色谱分析研究.
  • 基金资助:

    国家自然科学基金(批准号:29775002)

Determination of Estrodiol by Capillary Electrophoretic Immunoassay with Thermally Reversible Hydrogel

SU Ping, WANG Yong-Cheng, ZHANG Xin-Xiang, SUN Ling, CHANG Wen-Bao   

  1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
  • Received:2000-01-06 Online:2000-06-24 Published:2000-06-24

PDF (PC)

被引次数

46

关键词: 水凝胶, 毛细管电泳, 激光诱导荧光, 免疫分析, 雌二醇

Abstract: In this paper, a kind of thermally linear polymer, poly-N-isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. Auseful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pHof buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mLand 0.1—10 ng/mL.

Key words: Hydrogel, Capillary electrophoresis, Laser induced fluorescence, Immunoassay, Estradiol

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