高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (2): 232-235.

• 论文 • 上一篇    下一篇

流动型微流控PCR扩增芯片的研究

刘金华, 殷学锋, 徐光明, 方肇伦, 陈怀增   

  1. 浙江大学化学系微分析系统研究所, 杭州 310028
  • 收稿日期:2001-12-29 出版日期:2003-02-24 发布日期:2003-02-24
  • 通讯作者: 殷学锋(1945年生),男,教授,博士生导师,主要从事微流控分析研究.E-mial:cheyinxf@mail.hz.zj.cn
  • 基金资助:

    国家自然科学基金重大项目(批准号:20299030);浙江省自然科学基金(批准号:202078)资助

Studies on a Microfluidic Chip Based on the Continuous Flow PCR Amplification System

LIU Jin-Hua, YIN Xue-Feng, XU Guang-Ming, FANG Zhao-Lun, CHEN Huai-Zeng   

  1. Institute of Microanalysis System, Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2001-12-29 Online:2003-02-24 Published:2003-02-24

摘要: 组装了由注射泵进样系统、微流控芯片和三温区加热器组成的流动型PCR扩增系统,该系统具有扩增速度快、交叉污染小、芯片可重复使用和操作方便等特点.优化了芯片厚度、隔热材料和流速等影响PCR扩增的因素.在4.9min内经24个循环成功地扩增了浓度为1ng/100μL的λ-DNA(500bp).

关键词: PCR扩增, 微流控芯片, DNA

Abstract: A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work. The system is capable of performing continuous PCR amplification with a low carryover between neighboring samples. The substrate thickness and thermal insulation of the temperature control unit were optimized. A 24-cycle PCR amplification of a 500-base pair fragment from λ-DNA was successfully performed with a total reaction time of 4 9 min.

Key words: PCR amplification, Microfluidic chip, DNA

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